Morphological Observations of Frozen-Thawed Spermatozoa of Southern Minke Whales (Balaenoptera acutorostrata)
Morphological Observations of Frozen-Thawed Spermatozoa of Southern Minke Whales (Balaenoptera acutorostrata)
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南小须鲸（Balaenoptera acutorostrata）冻融精子的形态观察
DOI：
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
S. Ohsumi
中科院分区：
文献类型：
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作者：
T. Mogoe;Y. Fukui;H. Ishikawa;S. Ohsumi
对南方小须鲸精子冷冻后的形态、活力和存活率进行了研究。本研究中使用的鲸鱼是在1994年12月至1995年2月期间根据南极特别许可证为日本鲸鱼研究方案捕获的。将从11头鲸鱼输精管中回收的每个精子样品用Tris（300 mM）-柠檬酸盐（94.75 mM）-葡萄糖（27.75 mM）-蛋黄（15%; v/v）-甘油（5%; v/v）稀释剂稀释至1：9的比例。将稀释的样品在5 ℃下逐渐冷却2小时，在-80 ℃下冷冻并保存在液氮中。解冻后，主观评价精子活力。用伊红-黑色素染色法测定精子活力。精子畸形和头部形态进行了评估，石炭酸品红染色，使用相差显微镜。使用经校准的投影显微镜测量精子尺寸。精子活力和存活率分别为1.0%至20%（11个样本中的7个）和1.0%至11.6%。精子浓度范围为13 - 585.3 × 106个精子/ml。精子形态异常的平均比例为84.0 ± 6.7%。精子头部有7种形态类型，最常见的是圆锥形和椭圆形。精子全长56.7 ± 0.5 μm，精子头长5.2 ± 0.1 μm，宽3.0 ± 0.0 μm，精子尾长51.7 ± 0.5 μm。
The morphology, motility and viability of frozen-thawed southern minke whale (Balaenoptera acutorostrata) spermatozoa was evaluated. The whales used in this study were captured during the period from December 1994 to February 1995 for the Japanese Whale Research Programme under Special Permit in the Antarctic. Each sperm sample recovered from the vasa deferentia of eleven whales was diluted to a 1:9 ratio with Tris (300 mM)-citrate (94.75 mM)-glucose (27.75 mM)-egg yolk (15%; v/v)-glycerol (5%; v/v) diluents. The diluted samples were cooled gradually at 5 C for 2 h, frozen at -80 C and kept in liquid nitrogen. After thawing, sperm motility was evaluated subjectively. Sperm viability was assessed by eosin-nigrosin staining. Sperm abnormality and head morphology were assessed by carbol-fuchsin staining using phase-contrast microscopy. Sperm dimensions were measured using a calibrated projection microscope. Sperm motility and viability respectively varied from 1.0 to 20% (7 out of 11 samples), and 1.0 to 11.6%. Sperm concentration ranged from 13 to 585.3 × 106 spermatozoa/ml. The mean proportion of morphologically abnormal spermatozoa was 84.0 ± 6.7%. Seven morphotypes of sperm heads were observed, and the most common types were conical and elliptic in shape. The mean total length of spermatozoa was 56.7 ± 0.5 μm, with their sperm heads measuring 5.2 ± 0.1 μm for length and 3.0 ± 0.0 μm for width; the mean length of sperm tail was 51.7 ± 0.5 μm.
DOI：
10.1530/jrf.0.0630509
发表时间：
1981-11
期刊：
Journal of reproduction and fertility
影响因子：
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作者：
A. D. Fleming;R. Yanagimachi;H. Yanagimachi
通讯作者：
A. D. Fleming;R. Yanagimachi;H. Yanagimachi