Two pathogenesis-related proteins interact with leucine-rich repeat proteins to promote Alternaria leaf spot resistance in apple.
Two pathogenesis-related proteins interact with leucine-rich repeat proteins to promote Alternaria leaf spot resistance in apple.
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DOI：
10.1038/s41438-021-00654-4
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发表时间：
2021-10-01
影响因子：
8.7
通讯作者：
Li T
中科院分区：
文献类型：
--
作者：
Zhang Q;Xu C;Wei H;Fan W;Li T
苹果链格孢叶斑病（Alternaria leaf spot）是由苹果链格孢（Alternaria alternata f.）sp. mali（也称为A.马里）是一种毁灭性疾病，造成巨大的经济损失。我们先前已经确定，抗性（R）蛋白MdRNL 2含有卷曲螺旋、核苷酸结合和富含亮氨酸重复（CCR-NB-LRR）结构域，与另一种CCR-NB-LRR蛋白MdRNL 6相互作用，形成MdRNL 2-MdRNL 6复合物，赋予对A的抗性。马里.在这里，为了研究MdRNL 2-MdRNL 6复合物的功能，我们鉴定了两种与MdRNL 2相互作用的新的发病机制相关（PR）蛋白，MdPR 10 -1和MdPR 10 -2。酵母双杂交（Y2 H）分析和双分子荧光互补（BiFC）分析证实MdPR 10 -1和MdPR 10 -2与MdRNL 2和MdRNL 6在富含亮氨酸的重复结构域处相互作用。瞬时表达试验表明，MdPR 10 -1和MdPR 10 -2的积累增强了苹果对4个菌株的抗性。我们测试的苹果：ALT 1，GBYB 2，BXSB 5和BXSB 7。体外抗真菌活性测定表明，这两种蛋白质有助于通过抑制真菌生长的链格孢叶斑病抗性。我们的数据提供了一种新的调节机制，其中MdRNL 2和MdRNL 6与MdPR 10 -1和MdPR 10 -2相互作用，以抑制真菌生长，从而有助于苹果斑点落叶病抗性的证据。这两个新的PR蛋白的鉴定将有助于苹果抗真菌病害育种。
Alternaria leaf spot in apple (Malus x domestica), caused by the fungal pathogen Alternaria alternata f. sp. mali (also called A. mali), is a devastating disease resulting in substantial economic losses. We previously established that the resistance (R) protein MdRNL2, containing a coiled-coil, nucleotide-binding, and leucine-rich repeat (CCR-NB-LRR) domain, interacts with another CCR-NB-LRR protein, MdRNL6, to form a MdRNL2–MdRNL6 complex that confers resistance to A. mali. Here, to investigate the function of the MdRNL2–MdRNL6 complex, we identified two novel pathogenesis-related (PR) proteins, MdPR10-1 and MdPR10-2, that interact with MdRNL2. Yeast two-hybrid (Y2H) assays and bimolecular fluorescence complementation (BiFC) assays confirmed that MdPR10-1 and MdPR10-2 interact with MdRNL2 and MdRNL6 at the leucine-rich repeat domain. Transient expression assays demonstrated that accumulation of MdPR10-1 and MdPR10-2 enhanced the resistance of apple to four strains of A. mali that we tested: ALT1, GBYB2, BXSB5, and BXSB7. In vitro antifungal activity assays demonstrated that both the proteins contribute to Alternaria leaf spot resistance by inhibiting fungal growth. Our data provide evidence for a novel regulatory mechanism in which MdRNL2 and MdRNL6 interact with MdPR10-1 and MdPR10-2 to inhibit fungal growth, thereby contributing to Alternaria leaf spot resistance in apple. The identification of these two novel PR proteins will facilitate breeding for fungal disease resistance in apple.
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影响因子：
5.3
作者：
Besbes, Fatma;Habegger, Ruth;Schwab, Wilfried
通讯作者：
Schwab, Wilfried
DOI：
10.1046/j.1432-1327.1998.2580794.x
发表时间：
1998-12-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Fujimoto, Y;Nagata, R;Hashimoto, Y
通讯作者：
Hashimoto, Y
影响因子：
4.5
作者：
Bulajic, A;Filajdic, N;Sutton, TB
通讯作者：
Sutton, TB
影响因子：
7.2
作者：
Hugot, K;Aimé, S;Galiana, E
通讯作者：
Galiana, E
影响因子：
6.5
作者：
Gachon, C;Saindrenan, P
通讯作者：
Saindrenan, P