Impact of adding glucose-coated water-soluble silver nanoparticles to the silkworm larval diet on silk protein synthesis and related properties
Impact of adding glucose-coated water-soluble silver nanoparticles to the silkworm larval diet on silk protein synthesis and related properties
复制标题
蚕幼虫饲料中添加葡萄糖包被的水溶性银纳米粒子对丝蛋白合成及相关特性的影响
DOI：
10.1080/09205063.2019.1692642
复制
发表时间：
2019-11
期刊：
影响因子：
--
通讯作者：
Xu Shi-Qing
中科院分区：
文献类型：
--
作者：
Zhang Yun-Hu;Shi Mei-Juan;Li Kai-Le;Xing Rui;Chen Zhuo-Hua;Chen Xue-Dong;Wang Yong-Feng;Liu Xiao-Fei;Liang Xin-Yin;Sima Yang-Hu;Xu Shi-Qing
摘要丝素蛋白材料的生物改性在纺织品、生物医用材料及其它工业材料中有着广泛的应用。通过将纳米颗粒添加到家蚕幼虫饲料中来将纳米颗粒并入丝素蛋白的生物合成中是经济的。这种策略可能导致改性丝的快速稳定生产。采用葡萄糖包裹的银纳米粒子（AgNPs）改善其生物相容性，并通过饲养法将AgNPs有效地掺入蚕丝中。用银纳米粒子喂养的幼虫产生的蚕丝具有显着改善的抗菌性能和改变的蚕丝二级结构。不同剂量的银纳米颗粒对丝蛋白的生长和合成都有积极和消极的影响。与饲喂高浓度的2.00%AgNPs相比，饲喂低浓度的0.02%AgNPs和中等浓度的0.20%AgNPs的幼虫具有更高的AgNPs向蚕丝的转移效率。此外，所产生的丝纤维的伸长率和拉伸强度也显著增加，具有更大的哺乳动物细胞相容性。在家蚕饲料中添加适量的银纳米粒子，可以促进蚕丝蛋白的合成，提高蚕丝蛋白的机械性能，改善蚕丝蛋白的抗菌性能，抑制革兰氏阴性菌的存在。图形摘要摘要亮点蚕饲料中的AgNP可以有效地掺入蚕丝中。膳食AgNPs可以产生具有改变的机械性能的抗菌丝。低浓度的银纳米颗粒可以促进丝素蛋白的合成。
Abstract Biological modifications of the silk fibroin (silk) material have broad applications in textiles, biomedical materials and other industrial materials. It is economical to incorporate nanoparticles to the biosynthesis of silk fibroin by adding them to silkworm larval diets. This strategy may result in the rapid stable production of modified silk. Glucose-coated silver nanoparticles (AgNPs) were used to improve the AgNPs’ biocompatibility, and the AgNPs were efficiently incorporated into silk by feeding. Larvae fed with AgNPs produced silk with significantly improved antibacterial properties and altered silk secondary structures. Both positive and negative effects on the growth and synthesis of silk proteins were observed after different AgNPs doses. Larvae feeding with low concentration of 0.02% and medium 0.20% AgNPs have greater transfer efficiencies of AgNPs to silk compared with feeding high concentration of 2.00% AgNPs. In addition, the elongation and tensile strength of the produced silk fibers were also significantly increased, with greater mammalian cell compatibility. The appropriate AgNPs concentration in the diet of silkworms can promote the synthesis of silk proteins, enhance their mechanical properties, improve their antibacterial property and inhibit the presence of Gram-negative bacteria. Graphical Abstract HIGHLIGHTS AgNPs in silkworm diets can be efficiently incorporated into silk. Dietary AgNPs can produce antibacterial silk with altered mechanical properties. A low dietary AgNPs concentration can enhance silk fibroin synthesis.
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影响因子：
3
作者：
Gulrajani, M. L.;Gupta, Deepti;Muthu, S. G.
通讯作者：
Muthu, S. G.
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影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
19
作者：
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通讯作者：
T. Iizuka;H. Sezutsu;K. Tatematsu;I. Kobayashi;N. Yonemura;K. Uchino;Ken-ichi Nakajima;Katsura Kojima;C. Takabayashi;H. Machii;Katsushige Yamada;H. Kurihara;T. Asakura;Y. Nakazawa;A. Miyawaki;S. Karasawa;Hatsumi Kobayashi;Junji Yamaguchi;Nobuo Kuwabara;Takashi Nakamura;K. Yoshii;T. Tamura
DOI：
10.1016/j.jphotobiol.2017.07.001
发表时间：
2017-08
期刊：
Journal of photochemistry and photobiology. B, Biology
影响因子：
--
作者：
P. Velmurugan;Jae-In Kim;Kangmin Kim;Jung-Hee Park;Kui-jae Lee;Woo-Suk Chang;Yool‐Jin Park;Min Cho-Min
通讯作者：
P. Velmurugan;Jae-In Kim;Kangmin Kim;Jung-Hee Park;Kui-jae Lee;Woo-Suk Chang;Yool‐Jin Park;Min Cho-Min