Novel splice variants of PACAP gene in mouse cerebellar granule cells
Novel splice variants of PACAP gene in mouse cerebellar granule cells
复制标题
小鼠小脑颗粒细胞 PACAP 基因的新剪接变体
作者：
A. Tabuchi;Motoko Koizumi;M. Tsuda
小鼠垂体腺苷环化酶激活多肽(PACAP)基因由两个交替的外显子(1A和1B)和包括编码区在内的四个外显子组成。通过使用替代外显子，可以产生三个转录本。然而，在本报告中，我们发现在小鼠小脑颗粒细胞(CGCs)中至少产生了五种前PACAP转录本，其中包括两种新类型的转录本，它们的表达是由钙离子通过L型电压依赖性钙通道内流诱导的。剪接发生在外显子1A的3‘-供体部位和外显子1B的两个新的内部5’-受体部位之间。这些发现表明，前ProPACAP转录本是由5‘-非翻译区的复杂使用以一种活性依赖的方式产生的。
The mouse pituitary adenylate cyclase activating polypeptide (PACAP) gene consists of two alternative exons (1A and 1B) and four exons including the coding region. By the usage of alternative exons, three transcripts can be produced. In this report, however, we found that at least five preproPACAP transcripts, including two novel types, were produced in mouse cerebellar granule cells (CGCs) and their expression was induced by the Ca2+ influx through L-type voltage-dependent Ca2+ channels. The splicing occurred between the 3′-donor site of exon 1A and the two novel internal 5′-acceptor sites of exon 1B. These findings suggest that preproPACAP transcripts are produced by the complicated usage of the 5′-untranslated region in an activity-dependent manner.
影响因子：
21.1
作者：
D. Vaudry;B. Gonzalez;M. Basille;L. Yon;A. Fournier;H. Vaudry
通讯作者：
D. Vaudry;B. Gonzalez;M. Basille;L. Yon;A. Fournier;H. Vaudry
DOI：
10.1016/0006-291x(89)91757-9
发表时间：
1989-10-16
影响因子：
3.1
作者：
MIYATA, A;ARIMURA, A;COY, DH
通讯作者：
COY, DH