A Facile Approach for Rapid Prototyping of Microneedle Molds, Microwells and Micro-Through-Holes in Various Substrate Materials Using CO(2) Laser Drilling.
A Facile Approach for Rapid Prototyping of Microneedle Molds, Microwells and Micro-Through-Holes in Various Substrate Materials Using CO(2) Laser Drilling.
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DOI：
10.3390/biomedicines8100427
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发表时间：
2020-10-18
期刊：
影响因子：
4.7
通讯作者：
Tu TY
中科院分区：
文献类型：
--
作者：
Chen YW;Chen MC;Wu KW;Tu TY
在过去的几十年里，CO2激光制造一直是快速和具有成本效益的微加工的可靠工具。虽然已经研究了广泛的工业和生物应用，但在各种激光参数和系统中制造的材料的选择通常会被其复杂的组合所混淆。我们在本文中提出了一个统一的程序，使用冲击CO2激光钻孔与一系列的激光参数，基板材料和各种生成的微观结构，使各种下游组织/细胞为基础的应用。重点是描绘激光钻孔效果不同的生物相容性材料和概念验证实用程序。首先，制造聚二甲基硅氧烷（PDMS）微针（MN）阵列模具以产生用于经皮药物递送的可溶性聚乙烯吡咯烷酮/聚乙烯醇（PVP/PVA）MN。第二，聚苯乙烯（PS）微孔在一个紧凑的阵列中进行优化，以形成大小控制的多细胞肿瘤球体（MCTS）。第三，盖玻片被穿孔以形成可用于捕获/定位细胞/球状体的微孔。第四，在PS中创建通孔被验证为创建促进悬滴阵列中的介质交换的通道的可访问方法，并且作为用于MCTS的生长和药物筛选的有益工具。
CO2 laser manufacturing has served as an enabling and reliable tool for rapid and cost-effective microfabrication over the past few decades. While a wide range of industrial and biological applications have been studied, the choice of materials fabricated across various laser parameters and systems is often confounded by their complex combinations. We herein presented a unified procedure performed using percussion CO2 laser drilling with a range of laser parameters, substrate materials and various generated microstructures, enabling a variety of downstream tissue/cellular-based applications. Emphasis is placed on delineating the laser drilling effect on different biocompatible materials and proof-of-concept utilities. First, a polydimethylsiloxane (PDMS) microneedle (MN) array mold is fabricated to generate dissolvable polyvinylpyrrolidone/polyvinyl alcohol (PVP/PVA) MNs for transdermal drug delivery. Second, polystyrene (PS) microwells are optimized in a compact array for the formation of size-controlled multicellular tumor spheroids (MCTSs). Third, coverglass is perforated to form a microaperture that can be used to trap/position cells/spheroids. Fourth, the creation of through-holes in PS is validated as an accessible method to create channels that facilitate medium exchange in hanging drop arrays and as a conducive tool for the growth and drug screenings of MCTSs.
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影响因子：
6.1
作者：
Chen, Chang-Yu;Tu, Ting-Yuan;Wo, Andrew M.
通讯作者：
Wo, Andrew M.
影响因子：
--
作者：
Raghavan S;Mehta P;Horst EN;Ward MR;Rowley KR;Mehta G
通讯作者：
Mehta G
影响因子：
3.8
作者：
Gunness, Patrina;Mueller, Daniel;Noor, Fozia
通讯作者：
Noor, Fozia
纳米技术医疗和制药设备中的微米和纳米制造方法。
影响因子：
8
作者：
Betancourt, Tania;Brannon-Peppas, Lisa
通讯作者：
Brannon-Peppas, Lisa
DOI：
10.2147/dddt.s44401
发表时间：
2013
期刊：
Drug design, development and therapy
影响因子：
--
作者：
Hong X;Wei L;Wu F;Wu Z;Chen L;Liu Z;Yuan W
通讯作者：
Yuan W