Automated microbeam observation environment for biological analysis-Custom portable environmental control applied to a vertical microbeam system.
Automated microbeam observation environment for biological analysis-Custom portable environmental control applied to a vertical microbeam system.
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DOI：
10.1016/j.snb.2016.08.076
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发表时间：
2017-03
期刊：
影响因子：
--
通讯作者：
Kirkby KJ
中科院分区：
文献类型：
--
作者：
England MJ;Bigelow AW;Merchant MJ;Velliou E;Welch D;Brenner DJ;Kirkby KJ
垂直微光束（VMB）用于用低MeV能量离子照射单个细胞。使用VMBs照射细胞需要将细胞从培养箱中取出；由于培养箱外的二氧化碳浓度、温度和相对湿度较低，这会引起细胞的生理变化。因此，对于需要长时间照射和观察细胞的实验，提供一个可控的环境是很重要的。用于VMB系统的显微镜的高度定制性质意味着没有商用的环境控制的VMB系统显微镜系统。用于生物分析的自动微束观察环境（AMOEBA）是一种高度灵活的模块化环境控制系统，用于在VMB末端创建培养条件。AMOEBA利用了最近的“创客”运动来创建一个开源控制系统，用户可以轻松配置该系统，以满足他们的控制需求，甚至超出了VMB应用程序。当应用于控制细胞介质温度，二氧化碳浓度和VMB上的相对湿度的任务时，它创造了一个稳定的环境，允许细胞在VMB的末端在36小时内繁殖，提供了低成本（成本低于2700美元），可定制的替代商业延时显微镜系统。变形虫增加了vmb的潜力，探索辐射对单细胞的长期影响，为vmb开辟了新的研究领域。
Vertical Microbeams (VMB) are used to irradiate individual cells with low MeV energy ions. The irradiation of cells using VMBs requires cells to be removed from an incubator; this can cause physiological changes to cells because of the lower CO2 concentration, temperature and relative humidity outside of the incubator. Consequently, for experiments where cells require irradiation and observation for extended time periods, it is important to provide a controlled environment. The highly customised nature of the microscopes used on VMB systems means that there are no commercially available environmentally controlled microscope systems for VMB systems. The Automated Microbeam Observation Environment for Biological Analysis (AMOEBA) is a highly flexible modular environmental control system used to create incubator conditions on the end of a VMB. The AMOEBA takes advantage of the recent “maker” movement to create an open source control system that can be easily configured by the user to fit their control needs even beyond VMB applications. When applied to the task of controlling cell medium temperature, CO2 concentration and relative humidity on VMBs it creates a stable environment that allows cells to multiply on the end of a VMB over a period of 36 h, providing a low-cost (costing less than $2700 to build), customisable alternative to commercial time-lapse microscopy systems. AMOEBA adds the potential of VMBs to explore the long-term effects of radiation on single cells opening up new research areas for VMBs.
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影响因子：
3.7
作者：
Lynch AE;Triajianto J;Routledge E
通讯作者：
Routledge E
DOI：
10.1016/j.nima.2009.08.041
发表时间：
2009-10-11
影响因子：
1.4
作者：
Randers-Pehrson, Gerhard;Johnson, Gary W.;Marino, Stephen A.;Xu, Yanping;Dymnikov, Alexander D.;Brenner, David J.
通讯作者：
Brenner, David J.
DOI：
10.1073/pnas.94.8.3765
发表时间：
1997-04-15
影响因子：
11.1
作者：
Hei, TK;Wu, LJ;RandersPehrson, G
通讯作者：
RandersPehrson, G
影响因子：
4
作者：
Seyfried, Guenter;Pei, Lei;Schmidt, Markus
通讯作者：
Schmidt, Markus
影响因子：
1.5
作者：
Bigelow, Alan W.;Brenner, David J.;Randers-Pehrson, Gerhard
通讯作者：
Randers-Pehrson, Gerhard