Different responses to glucose overload between two strains of largemouth bass (Micropterus salmoides).
Different responses to glucose overload between two strains of largemouth bass (Micropterus salmoides).
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DOI：
10.3389/fphys.2022.1010633
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发表时间：
2022
影响因子：
4
通讯作者：
Li, Shengjie
中科院分区：
文献类型：
--
作者：
Lei, Caixia;Xie, Yujing;Song, Hongmei;Jiang, Peng;Du, Jinxing;Li, Shengjie
为了提高大口黑鲈（Micropterus salmoides）对葡萄糖的利用能力，比较了两种菌株（Y：繁殖菌株，W：野生菌株）在注射葡萄糖（1.67 g/kg）后0、6、12和24 h对葡萄糖过载的反应。结果显示，Y株（<12 h）血浆葡萄糖恢复速度快于W株（12 h），且Y株在注射后6 h内胰岛素分泌较多。Y菌株甘油三酯（TG）和低密度脂蛋白-胆固醇（VLDL-CH）含量升高，在12 h达到峰值，然后下降，而W菌株的TG含量不受影响，VLDL-CH含量下降。肝脏和肌肉脂肪酸合成酶、肝脏x受体-1和甾醇调节元件结合蛋白的表达与TG含量变化一致。两菌株肝脏和肌肉糖原含量的变化趋势与糖原合成酶基因的变化趋势相似，均先升高后下降，并在6和12 h达到峰值。Y菌株肝脏和肌肉磷酸果糖激酶和丙酮酸激酶的表达量升高，在12 h达到峰值。两菌株肝脏和肌肉磷酸烯醇丙酮酸羧激酶和葡萄糖-6-磷酸酶mRNA表达量均增强，并在24 h达到峰值，肝脏异柠檬酸脱氢酶-1和α-酮戊二酸脱氢酶复合物表达量先下降后上升，在12和24 h达到峰值。W菌株肌肉中两个基因的表达趋势相似。两种菌株的转录组结果鉴定出七个共同的高血糖抵抗功能基因，这些基因参与昼夜节律途径，这是应对高血糖的关键途径。此外，在两种菌株之间鉴定出48个差异基因，这些基因在tgf - β和细胞周期信号通路中富集，表明这些通路可能是影响葡萄糖过载差异反应的关键因素。我们对两株沙门草的葡萄糖过载分子响应进行了全面比较，结果可以为沙门草基于优势的先存性状提高葡萄糖利用能力提供有希望的策略。
In order to improve the glucose utilization capacity of largemouth bass (Micropterus salmoides), responses to glucose overload between two strains (Y: breeding strain; W: wild strain) were compared at 0, 6, 12, and 24 h after glucose injection (1.67 g/kg). The data revealed that plasma glucose in the Y strain (<12 h) recovered faster than in the W strain (12 h), with the Y strain secreted more insulin within 6 h post-injection. Triglyceride (TG) and low-density lipoprotein-cholesterol (VLDL-CH) content in the Y strain increased, peaking at 12 h, then decreased, whereas the W strain’s TG content was not affected and VLDL-CH content decreased. The hepatic and muscular fatty acid synthetase, liver x receptor-1, and sterol regulatory element-binding protein expressions were consistent with the TG content change. Both strains’ liver and muscle glycogen contents exhibited similar trends to that of the glycogen synthase gene—increasing, then declining, and peaking at 6 and 12 h. The expression levels of hepatic and muscular phosphofructokinase and pyruvate kinase in the Y strain increased, peaking at 12 h. In the W strain, they were suppressed and reached the minimum at 24 h. The mRNA levels of hepatic and muscular phosphoenolpyruvate carboxykinase and glucose-6-phosphatase were enhanced and peaked at 24 h in both strains, hepatic isocitrate dehydrogenase-1, and α-ketoglutarate dehydrogenase complex expression increased after declining, peaking at 12 and 24 h. Two genes in the W strain’s muscles showed a similar trend. Both strains’ transcriptome results identified seven common functional genes for resistance to hyperglycemia that were involved in the circadian rhythm pathway, which is a suggested key pathway for coping with hyperglycemia. Furthermore, 48 differential genes were identified between the two strains, and these genes were enriched in the TGF-beta and cell cycle signaling pathways, indicating that these pathways may be key factors affecting the differential responses to glucose overload. We conducted a comprehensive comparison of glucose overload molecular responses between two strains of M. salmoides, and the results can provide a promising strategy to improve the glucose utilization capacity of M. salmoides based on advantageous pre-existing traits.
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影响因子：
2.8
作者：
Kamalam, Biju Sam;Medale, Francoise;Panserat, Stephane
通讯作者：
Panserat, Stephane
影响因子：
4.8
作者：
Diaz, Monica;Antonescu, Costin N.;Planas, Josep V.
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Planas, Josep V.
DOI：
10.1016/j.cbpb.2022.110715
发表时间：
2022-01-13
影响因子：
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作者：
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Panserat, Stephane
影响因子：
2.7
作者：
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通讯作者：
Medale, Francoise