Sperm, but not oocyte, DNA methylome is inherited by zebrafish early embryos.
Sperm, but not oocyte, DNA methylome is inherited by zebrafish early embryos.
复制标题
斑马鱼早期胚胎遗传了精子而非卵母细胞的DNA甲基化组
DOI：
10.1016/j.cell.2013.04.041
复制
发表时间：
2013-05-09
期刊：
影响因子：
64.5
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Jiang L;Zhang J;Wang JJ;Wang L;Zhang L;Li G;Yang X;Ma X;Sun X;Cai J;Zhang J;Huang X;Yu M;Wang X;Liu F;Wu CI;He C;Zhang B;Ci W;Liu J
5-甲基胞嘧啶是一种主要的表观遗传修饰，有时被称为“第五核苷酸”。然而，我们对后代如何从父母那里继承 DNA 甲基化组的了解有限。我们生成了九个单碱基分辨率 DNA 甲基化组，包括斑马鱼配子和早期胚胎。与精子相比，卵母细胞甲基化显着低甲基化。引人注目的是，父本 DNA 甲基化模式在整个早期胚胎发生过程中得以维持。母体 DNA 甲基化模式一直维持到 16 细胞阶段。然后，卵母细胞甲基化组通过细胞分裂逐渐被丢弃，并逐渐重新编程为类似于精子甲基化组的模式。母体DNA中的被动去甲基化率和从头甲基化率相似。到囊胚中期，胚胎的甲基化组实际上与精子的甲基化组相同。此外，精子甲基化组的遗传促进了胚胎发生的表观遗传调控。因此，除了DNA序列之外，精子DNA甲基化组也在斑马鱼早期胚胎中遗传。
5-methylcytosine is a major epigenetic modification that is sometimes called “the fifth nucleotide.” However, our knowledge of how offspring inherit the DNA methylome from parents is limited. We generated nine single-base resolution DNA methylomes, including zebrafish gametes and early embryos. The oocyte methylome is significantly hypomethylated compared to sperm. Strikingly, the paternal DNA methylation pattern is maintained throughout early embryogenesis. The maternal DNA methylation pattern is maintained until the 16-cell stage. Then, the oocyte methylome is gradually discarded through cell division and is progressively reprogrammed to a pattern similar to that of the sperm methylome. The passive demethylation rate and the de novo methylation rate are similar in the maternal DNA. By the midblastula stage, the embryo’s methylome is virtually identical to the sperm methylome. Moreover, inheritance of the sperm methylome facilitates the epigenetic regulation of embryogenesis. Therefore, besides DNA sequences, sperm DNA methylome is also inherited in zebrafish early embryos.
登录
查看更多内容
影响因子：
46.9
作者：
Ball, Madeleine P.;Li, Jin Billy;Gao, Yuan;Lee, Je-Hyuk;LeProust, Emily M.;Park, In-Hyun;Xie, Bin;Daley, George Q.;Church, George M.
通讯作者：
Church, George M.
影响因子：
64.5
作者：
Calarco JP;Borges F;Donoghue MT;Van Ex F;Jullien PE;Lopes T;Gardner R;Berger F;Feijó JA;Becker JD;Martienssen RA
通讯作者：
Martienssen RA
在小鼠表观遗传敏感等位基因上DNA甲基化的动态重编程。
影响因子：
4.5
作者：
Blewitt ME;Vickaryous NK;Paldi A;Koseki H;Whitelaw E
通讯作者：
Whitelaw E
影响因子：
64.5
作者：
Cavalli, G;Paro, R
通讯作者：
Paro, R
DOI：
10.1126/science.1190614
发表时间：
2010-10-29
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Feng S;Jacobsen SE;Reik W
通讯作者：
Reik W