Cold-induced secondary dormancy and its regulatory mechanisms in Beta vulgaris.
Cold-induced secondary dormancy and its regulatory mechanisms in Beta vulgaris.
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DOI：
10.1111/pce.14264
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发表时间：
2022-04
影响因子：
7.3
通讯作者：
Leubner-Metzger, Gerhard
中科院分区：
文献类型：
--
作者：
Hourston, James E.;Steinbrecher, Tina;Chandler, Jake O.;Perez, Marta;Dietrich, Katrin;Tureckova, Veronika;Tarkowska, Danuse;Strnad, Miroslav;Weltmeier, Fridtjof;Meinhard, Juliane;Fischer, Uwe;Fiedler-Wiechers, Karin;Ignatz, Michael;Leubner-Metzger, Gerhard
关键词：
土壤种子库中种子的动态行为取决于它们作为复杂的环境传感器通过休眠机制调节发芽敏感阈值的能力。在这里，我们表明，在低温下长时间孵育甜菜果实（在5°C下冷却，通常被认为可以解除许多物种的种子休眠）可以诱导明显非休眠作物物种的次生非深度生理休眠。这种低温诱导的次生休眠的生理和生物物理机制包括低温诱导的脱落酸在种子中的积累，胚生长潜力的降低和覆盖胚根的胚乳弱化的阻碍。转录组分析显示不同的基因表达模式在不同的温度制度和次生休眠诱导和维护。低温导致胚细胞伸长生长和胚乳弱化所需的细胞壁重塑蛋白基因的表达减少，以及种子成熟基因的表达增加，如胚胎发生后期丰富的蛋白质。提出了一个将激素信号和主调节因子表达与种子休眠和成熟程序的温度控制相结合的模型。揭示冷诱导次生休眠的机制对于气候智能型农业和粮食安全具有重要意义。分散的种子和果实是环境温度的复杂传感器，并通过休眠来调整它们对环境的反应。在这里，我们揭示了分子和生物物理机制的低温诱导次生休眠在明显的非休眠甜菜作物种子。详细了解作物物种的二次休眠对于气候智能型农业、培育耐逆作物和可持续种子技术以减轻气候变化的负面影响非常重要。
The dynamic behaviour of seeds in soil seed banks depends on their ability to act as sophisticated environmental sensors to adjust their sensitivity thresholds for germination by dormancy mechanisms. Here we show that prolonged incubation of sugar beet fruits at low temperature (chilling at 5°C, generally known to release seed dormancy of many species) can induce secondary nondeep physiological dormancy of an apparently nondormant crop species. The physiological and biophysical mechanisms underpinning this cold‐induced secondary dormancy include the chilling‐induced accumulation of abscisic acid in the seeds, a reduction in the embryo growth potential and a block in weakening of the endosperm covering the embryonic root. Transcriptome analysis revealed distinct gene expression patterns in the different temperature regimes and upon secondary dormancy induction and maintenance. The chilling caused reduced expression of cell wall remodelling protein genes required for embryo cell elongation growth and endosperm weakening, as well as increased expression of seed maturation genes, such as for late embryogenesis abundant proteins. A model integrating the hormonal signalling and master regulator expression with the temperature‐control of seed dormancy and maturation programmes is proposed. The revealed mechanisms of the cold‐induced secondary dormancy are important for climate‐smart agriculture and food security. Dispersed seeds and fruits are sophisticated sensors of ambient temperature and adjust their responses to the environment via dormancy. Here we reveal the molecular and biophysical mechanisms of chilling‐induced secondary dormancy in apparently nondormant sugar beet crop seeds. Detailed knowledge of the secondary dormancy in crop species is important for climate‐smart agriculture, for breeding stress‐tolerant crops and sustainable seed technologies that mitigate the negative impacts of climate change.
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影响因子：
7.2
作者：
Dekkers, Bas J. W.;He, Hanzi;Bentsink, Leonie
通讯作者：
Bentsink, Leonie
影响因子：
6.9
作者：
Chen, F;Nonogaki, H;Bradford, KJ
通讯作者：
Bradford, KJ
影响因子：
4.9
作者：
Chhun, Tory;Chong, Suet Yen;Chua, Nam-Hai
通讯作者：
Chua, Nam-Hai
影响因子：
64.8
作者：
Dohm, Juliane C.;Minoche, Andre E.;Himmelbauer, Heinz
通讯作者：
Himmelbauer, Heinz
影响因子：
0.6
作者：
BANOVETZ, SJ;SCHEINER, SM
通讯作者：
SCHEINER, SM