Effects of Xylo-Oligosaccharides on Growth and Gut Microbiota as Potential Replacements for Antibiotic in Weaning Piglets.
Effects of Xylo-Oligosaccharides on Growth and Gut Microbiota as Potential Replacements for Antibiotic in Weaning Piglets.
复制标题
低聚木糖作为断奶仔猪抗生素的潜在替代品对生长和肠道微生物群的影响
DOI：
10.3389/fmicb.2021.641172
复制
发表时间：
2021
影响因子：
5.2
通讯作者：
Everaert N
中科院分区：
文献类型：
--
作者：
Chen Y;Xie Y;Zhong R;Liu L;Lin C;Xiao L;Chen L;Zhang H;Beckers Y;Everaert N
低聚木糖（Xylo-oligosaccharides，XOS）是一种常见的低聚糖，作为益生元被广泛应用。本研究旨在探讨低聚木糖（XOS）替代金霉素（CTC）对断奶仔猪生长、肠道形态、肠道菌群和后肠短链脂肪酸（SCFA）含量的影响。试验选用180头断奶仔猪，随机分为3组，即对照组（基础日粮，CON）、基础日粮中添加500 mg/kg（XOS 500）XOS和阳性对照组（基础日粮中添加100 mg/kg CTC），试验期28 d。与对照组相比，XOS 500组仔猪28日龄体重、1-28日龄平均日增重显著提高（P < 0.05），料重比显著降低（P < 0.05）。添加XOS 500显著提高了回肠绒毛高度和绒毛高度与隐窝深度的比值（P < 0.05）。绒毛高度：CTC治疗组回肠隐窝深度也增加（P < 0.05）。同时，添加XOS 500显著增加盲肠隐窝中杯状细胞的数量。高通量16 S rRNA基因测序显示回肠和盲肠之间的微生物组成存在明显差异。XOS 500补充显著增加细菌多样性。而CTC处理显著降低了微生物多样性（P < 0.05）。与CON组和CTC组相比，XOS 500组回肠和盲肠中乳酸杆菌的丰度显著增加（P < 0.01），而Clostridium_sensu_stricto_1、土氏志贺菌和Terrisporphinae的水平显著降低（P < 0.05）。此外，与CON组相比，日粮中添加XOS 500显著提高了盲肠中总短链脂肪酸、丙酸和丁酸的浓度，降低了乙酸的浓度（P < 0.05）。综上所述，日粮中添加XOS 500可以提高特定有益菌群的丰度，降低有害菌群的丰度，从而维持断奶仔猪肠道的形态结构，提高断奶仔猪的生长性能。因此，低聚木糖有可能作为现代畜牧业中断奶仔猪饲用抗生素的替代品。
Xylo-oligosaccharides (XOS) is a well-known kind of oligosaccharide and extensively applied as a prebiotic. The objective of this study was to investigate the effect of XOS supplementation substituting chlortetracycline (CTC) on growth, gut morphology, gut microbiota, and hindgut short chain fatty acid (SCFA) contents of weaning piglets. A total of 180 weaned piglets were randomly allocated to three treatments for 28 days, as follows: control group (basal diet, CON), basal diet with 500 mg/kg (XOS500) XOS, and positive control (basal diet with 100 mg/kg CTC). Compared with the CON group, the piglets in the XOS500 group improved body weight (BW) on days 28, average daily gain (ADG) and reduced feed: gain ratio during days 1–28 (P < 0.05). The XOS500 supplementation increased Villus height and Villus height: Crypt depth ratio in the ileum (P < 0.05). Villus Height: Crypt Depth of the ileum was also increased in the CTC treatment group (P < 0.05). Meanwhile, the XOS500 supplementation increased significantly the numbers of goblet cells in the crypt of the cecum. High-throughput 16S rRNA gene sequencing revealed distinct differences in microbial compositions between the ileum and cecum. XOS500 supplementation significantly increased the bacterial diversity. However, CTC treatment markedly reduced the microbial diversity (P < 0.05). Meanwhile, XOS500 supplementation in the diet significantly increased the abundance of Lactobacillus genus compared to the CON and CTC group in the ileum and cecum (P < 0.01), whereas the level of Clostridium_sensu_stricto_1, Escherichia-Shigella, and Terrisporobacter genus in the XOS500 group were markedly lower than the CON and CTC group (P < 0.05). In addition, dietary supplementation with XOS500 significantly increased the total short-chain fatty acids, propionate and butyrate concentrations and decreased the acetate concentration compared to the CON group in the cecum (P < 0.05). In summary, dietary supplemented with XOS500 could enhance specific beneficial microbiota abundance and decrease harmful microbiota abundance to maintain the structure of the intestinal morphology and improve growth performance of weaned piglets. Thus, XOS may potentially function as an alternative to in-feed antibiotics in weaned piglets in modern husbandry.
登录
查看更多内容
影响因子：
3.7
作者：
Cheng MP;Domingo MC;Lévesque S;Yansouni CP
通讯作者：
Yansouni CP
影响因子：
3.7
作者：
Cromwell, GL
通讯作者：
Cromwell, GL
DOI：
10.1099/ijsem.0.000450
发表时间：
2015-10-01
影响因子：
2.8
作者：
Deng, Yu;Guo, Xiang;Ruan, Zhiyong
通讯作者：
Ruan, Zhiyong
DOI：
10.1038/nrneph.2015.10
发表时间：
2015-03-01
期刊：
Nature reviews. Nephrology
影响因子：
--
作者：
Carney, Ellen F
通讯作者：
Carney, Ellen F
影响因子：
2.5
作者：
Callaway, T. R.;Edrington, T. S.;Nisbet, D. J.
通讯作者：
Nisbet, D. J.