Imaging of glutamate in the spinal cord using GluCEST.
Imaging of glutamate in the spinal cord using GluCEST.
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DOI：
10.1016/j.neuroimage.2013.03.072
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发表时间：
2013-08-15
期刊：
影响因子：
5.7
通讯作者：
Reddy, Ravinder
中科院分区：
文献类型：
--
作者：
Kogan, Feliks;Singh, Anup;Debrosse, Catherine;Haris, Mohammad;Cai, Kejia;Nanga, Ravi Prakash;Elliott, Mark;Hariharan, Hari;Reddy, Ravinder
谷氨酸（Glu）是脑和脊髓中最丰富的兴奋性神经递质。Glu的浓度在一系列影响脊髓的神经系统疾病中改变，包括多发性硬化症（MS）、肌萎缩性侧索硬化症（ALS）和脊髓损伤。目前可用的用于测量谷氨酸的磁共振光谱（MRS）方法仅限于低空间分辨率，这使得难以测量灰质和白色物质谷氨酸盐的差异。最近，研究表明，Glu在其胺（−NH2）和本体水质子（GluCEST）之间表现出浓度依赖性化学交换饱和转移（CEST）效应。在这里，我们证明了成像谷氨酸在脊髓中在7T使用GluCEST技术的可行性。来自健康人类志愿者（N=7）的结果显示，与白色物质（4.8 ± 0.4%）相比，来自灰质（6.6 ± 0.3%）的GluCESTasym显著更高（p<0.001）。潜在的重叠CEST信号从其他脊髓代谢物所观察到的GluCESTasym进行了讨论。这种非侵入性的方法可能开辟了在脊髓中对Glu进行体内成像并监测其在许多疾病条件下的变化的途径。
Glutamate (Glu) is the most abundant excitatory neurotransmitter in the brain and spinal cord. The concentration of Glu is altered in a range of neurologic disorders that affect the spinal cord including multiple sclerosis (MS), amyotrophic lateral sclerosis (ALS) and spinal cord injury. Currently available magnetic resonance spectroscopy (MRS) methods for measuring Glu are limited to low spatial resolution, which makes it difficult to measure differences in gray and white matter glutamate. Recently, it has been shown that Glu exhibits a concentration dependent chemical exchange saturation transfer (CEST) effect between its amine (−NH2) and bulk water protons (GluCEST). Here, we demonstrate the feasibility of imaging glutamate in the spinal cord at 7T using the GluCEST technique. Results from healthy human volunteers (N=7) showed a significantly higher (p<0.001) GluCESTasym from gray matter (6.6 ± 0.3%) compared to white matter (4.8 ± 0.4%). Potential overlap of CEST signals from other spinal cord metabolites with the observed GluCESTasym is discussed. This noninvasive approach potentially opens the way to image Glu in vivo in the spinal cord and to monitor its alteration in many disease conditions.
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影响因子：
3.3
作者：
Singh, Anup;Cai, Kejia;Haris, Mohammad;Hariharan, Hari;Reddy, Ravinder
通讯作者：
Reddy, Ravinder
影响因子：
2.2
作者：
Ward, KM;Aletras, AH;Balaban, RS
通讯作者：
Balaban, RS
影响因子：
14.5
作者：
Srinivasan, R;Sailasuta, N;Pelletier, D
通讯作者：
Pelletier, D
影响因子：
158.5
作者：
ROTHSTEIN, JD;MARTIN, LJ;KUNCL, RW
通讯作者：
KUNCL, RW
影响因子：
5.7
作者：
Inglese, Matilde;Rusinek, Henry;Gonen, Oded
通讯作者：
Gonen, Oded