High-fat diet-induced hypertension is associated with a proinflammatory T cell profile in male and female Dahl salt-sensitive rats.
High-fat diet-induced hypertension is associated with a proinflammatory T cell profile in male and female Dahl salt-sensitive rats.
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DOI：
10.1152/ajpheart.00389.2018
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发表时间：
2018-12-01
期刊：
影响因子：
--
通讯作者：
Sullivan JC
中科院分区：
文献类型：
--
作者：
Taylor LE;Gillis EE;Musall JB;Baban B;Sullivan JC
有证据表明，高脂肪饮食 (HFD) 对心血管结局的影响存在性别差异，雄性实验动物比雌性实验动物表现出更大的血压 (BP) 升高。免疫系统与 HFD 引起的血压升高有关，并且高血压中 T 细胞的激活存在性别差异。本研究的目的是确定 HFD 对雄性和雌性 Dahl 盐敏感 (DSS) 大鼠血压以及主动脉和肾 T 细胞谱的影响。我们假设，与雌性 DSS 大鼠相比，雄性 DSS 大鼠响应 HFD 后血压和 T 细胞浸润会出现更大的增加。通过尾套体积描记法测量血压，并通过流式细胞术分析 12 至 16 周龄正常脂肪饮食 (NFD) 或 HFD 的雄性和雌性 DSS 大鼠的主动脉和肾 T 细胞。四周的 HFD 使雄性和雌性 DSS 大鼠的血压升高程度相似。尽管雄性大鼠具有更多的促炎性 T 细胞，但两性的血压升高都伴随着 CD4+ T 细胞和辅助性 T (Th)17 细胞百分比的增加。 HFD 使两性大鼠肾脏 CD3+ 和 CD4+ T 细胞以及 Th17 细胞的百分比均有所增加，但雄性大鼠中 CD3+ T 细胞的增加幅度更大。 HFD 还降低了两性的主动脉和肾脏调节性 T 细胞的百分比，尽管无论饮食如何，雌性大鼠都比雄性大鼠保持更多的调节性 T 细胞。总之，雄性和雌性 DSS 大鼠均对 HFD 表现出血压敏感性；然而，介导 HFD 诱导的血压升高的机制可能有所不同，因为雄性大鼠的促炎性 T 细胞百分比比雌性大鼠表现出更大的增加。新的和值得注意的我们的研究表明，雄性和雌性 Dahl 盐敏感大鼠表现出与高脂肪饮食相似的血压升高以及主动脉和肾 T 细胞的增加。这些结果与研究形成鲜明对比，研究表明与雄性大鼠相比，雌性大鼠血压保持正常和/或上调调节性 T 细胞对高血压刺激的反应。我们的数据表明，4 周的高脂肪饮食对 Dahl 盐敏感大鼠的 T 细胞谱具有性别特异性影响。
Evidence supports a sex difference in the impact of a high-fat diet (HFD) on cardiovascular outcomes, with male experimental animals exhibiting greater increases in blood pressure (BP) than female experimental animals. The immune system has been implicated in HFD-induced increases in BP, and there is a sex difference in T-cell activation in hypertension. The goal of this study was to determine the impact of HFD on BP and aortic and renal T cell profiles in male and female Dahl salt-sensitive (DSS) rats. We hypothesized that male DSS rats would have greater increases in BP and T cell infiltration in response to a HFD compared with female DSS rats. BP was measured by tail-cuff plethysmography, and aortic and renal T cells were assessed by flow cytometric analysis in male and female DSS rats on a normal-fat diet (NFD) or HFD from 12 to 16 wk of age. Four weeks of HFD increased BP in male and female DSS rats to a similar degree. Increases in BP were accompanied by increased percentages of CD4+ T cells and T helper (Th)17 cells in both sexes, although male rats had more proinflammatory T cells. Percentages of renal CD3+ and CD4+ T cells as well as Th17 cells were increased in both sexes by the HFD, although the increase in CD3+ T cells was greater in male rats. HFD also decreased the percentage of aortic and renal regulatory T cells in both sexes, although female rats maintained more regulatory T cells than male rats regardless of diet. In conclusion, both male and female DSS rats exhibit BP sensitivity to a HFD; however, the mechanisms mediating HFD-induced increases in BP may be distinct as male rats exhibit greater increases in the percentage of proinflammatory T cells than female rats. NEW & NOTEWORTHY Our study demonstrates that male and female Dahl salt-sensitive rats exhibit similar increases in blood pressure to a high-fat diet and an increase in aortic and renal T cells. These results are in contrast to studies showing that female rats remain normotensive and/or upregulate regulatory T cells in response to hypertensive stimuli compared with male rats. Our data suggest that a 4-wk high-fat diet has sex-specific effects on the T cell profile in Dahl salt-sensitive rats.
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DOI：
10.1016/j.autneu.2014.11.006
发表时间：
2015-01
期刊：
Autonomic neuroscience : basic & clinical
影响因子：
--
作者：
Brooks VL;Shi Z;Holwerda SW;Fadel PJ
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血管紧张素II依赖性高血压的性别特异性T细胞调节。
DOI：
10.1161/hypertensionaha.114.03663
发表时间：
2014-09
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
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2012-06
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Arteriosclerosis, thrombosis, and vascular biology
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影响因子：
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Barhoumi, Tlili;Kasal, Daniel A.;Schiffrin, Ernesto L.
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DOI：
10.1124/jpet.102.045682
发表时间：
2003-04-01
影响因子：
3.5
作者：
Coatmellec-Taglioni, G;Dausse, JP;Ribière, C
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