Identification and expression analysis of APETALA1 homologues in poplar

Identification and expression analysis of APETALA1 homologues in poplar
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杨树APETALA1同源物的鉴定及表达分析

DOI:
10.1007/s11738-015-1805-z
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发表时间:
2015-02
影响因子:
2.6
通讯作者:
An XM
An XM
中科院分区:
生物学4区
文献类型:
--
作者:
Rao P;Gao Kai;Lei BQ;An XM

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aptala1在花从营养阶段到生殖阶段的转变和花的发育中起着至关重要的作用。本研究通过描述AP1基因结构和染色体定位,对杨树AP1同源基因进行了全面分析。分析了拟南芥AP1及杨树AP1同源物与其他AP1样蛋白的系统发育关系。采用RT-qPCR方法检测了PtAP1-1和PtAP1-2在毛白杨中的表达。表达谱相似,两个基因在生殖期均表现出高表达水平。花蕾中PtAP1-1和PtAP1-2的季节性表达谱表明,PtAP1-1和PtAP1-2在雌雄花蕾中的表达模式不同。PtAP1-1和PtAP1-2基因在两性花蕾中的转录水平变化趋势相似,但这两个基因在雄性花蕾中的表达高峰要早于雌性花蕾。本研究对今后杨树AP1同源物的功能分析具有一定的参考价值。
APETALA1 plays a crucial role in floral transition from vegetative to reproductive phase and in flower development. In this study, a comprehensive analysis of AP1 homologues in poplar was performed by describing the gene structure and chromosomal location. The phylogenetic relationship of the deduced amino acid sequences of Arabidopsis AP1 and AP1 homologues from Populus, to other AP1-like proteins was analyzed. The expression of PtAP1-1 and PtAP1-2 in Populus tomentosa was examined by RT-qPCR. Expression profiles were similar and both genes exhibited a high expression level in the reproductive phase. Seasonal expression profiles in floral buds indicated that the pattern of PtAP1-1 and PtAP1-2 expression in male and female floral buds was different. The trends of the PtAP1-1 and PtAP1-2 transcript levels in both sex floral buds were similar, but the peak of expression of the two genes in male buds was earlier than in female buds. This work would be of value to future functional analysis of AP1 homologues in poplar.
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