The mTOR cell signaling pathway is crucial to the long-term protective effects of ischemic postconditioning against stroke.
The mTOR cell signaling pathway is crucial to the long-term protective effects of ischemic postconditioning against stroke.
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mTOR 细胞信号通路对于缺血后处理对中风的长期保护作用至关重要。
DOI：
10.1016/j.neulet.2018.03.062
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发表时间：
2018-05-29
影响因子：
2.5
通讯作者：
Zhao H
中科院分区：
文献类型：
--
作者：
Wang P;Xie R;Cheng M;Sapolsky R;Ji X;Zhao H
Ischemic postconditioning (IPostC) protects against stroke, but few have studied the pathophysiological mechanisms of its long-term protective effects. Here, we investigated whether the mTOR pathway is involved in the long-term protective effects of IPostC. Stroke was induced in rats by distal middle cerebral artery occlusion (dMCAo) combined with 30 min of bilateral common carotid artery (CCA) occlusion, and IPostC was induced after the CCA release. Injury size and behavioral tests were measured up to 3 weeks post stroke. We used rapamycin and mTOR shRNA lentiviral vectors to inhibit mTOR activities, while S6K1 viral vectors, a main downstream mTOR gene, were used to promote mTOR activities. We found that rapamycin administration abolished the long-term protective effects of IPostC. In addition, IPostC promoted the presynaptic growth associated protein 43 (GAP-43) and the postsynaptic protein 95 (PSD-95) levels at 1 week post-stroke, which were reduced by rapamycin. Furthermore, rapamycin reduced phosphorylated mTOR (p-mTOR) protein levels measured at 3 weeks after stroke. These results were confirmed by mTOR shRNA transfection. Moreover, we found that injection of S6K1 viral vectors promoted GAP-43 and PSD-95 protein levels. We conclude that mTOR may play a crucial, protective role in brain damage after stroke and contribute to the protective effects of IPostC.
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影响因子：
4.8
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Keith, Dove;El-Husseini, Alaa
通讯作者：
El-Husseini, Alaa
影响因子：
3.7
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Wang, Ying
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4.2
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