A novel genotyping method for rapid identification of the Le gene to select patients for diagnosis with CA19-9.
A novel genotyping method for rapid identification of the Le gene to select patients for diagnosis with CA19-9.
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一种新的基因分型方法，用于快速鉴定 Le 基因，以选择诊断 CA19-9 的患者。
DOI：
10.1016/j.cca.2022.11.006
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Yazawa S.
中科院分区：
文献类型：
--
作者：
Sano R;Yokobori T;Harimoto N;Saeki H;Kominato Y;Shirabe K;Yazawa S.
背景CA 19 -9的抗原决定簇由刘易斯血型系统中Legene编码的α 1，3/4岩藻糖基转移酶合成。方法应用一组正义和生物素标记的反义引物及与等位基因中G59结合的肽核酸钳，进行LeGene特异性PCR检测c59 T>G。与链霉亲和素包被的蓝色乳胶珠混合后，PCR产物被开发上一条互补的标签寡核苷酸theLegene特异性amplicon是immobilized.ResultsWhen的PCR产物被开发上的条，一条清晰的线被迅速观察到在Le阳性，但不是在Le阴性的个人。与此相反，大量Lewis阴性表型的癌症患者被发现具有CA 19 -9，而他们的基因型被特异性地分型为Le/-。在315例恶性肿瘤患者中，未发现刘易斯基因型与CA 19 -9水平的矛盾结果。该方法似乎与选择诊断为CA 19 -9的癌症患者相关。
BackgroundThe antigenic determinant of CA19-9 is synthesized by the α1,3/4fucosyltransferase encoded by theLegene in the Lewis blood group system. Accordingly, a diagnosis with CA19-9 is not appropriate forLe-negative patients who possess theLegene-mutatedlealleles homozygously.MethodsALegene-specific PCR was undertaken to determine c59T>G by using a set of tag-sense and biotin-labeled anti-sense primers and a peptide nucleic acid-le-clamp which bound to G59 in thelealleles. Following mixing with streptavidin-coated blue latex beads, the PCR products were developed on a strip on which the complementary tag oligonucleotide to theLegene-specific amplicon was immobilized.ResultsWhen the PCR products were developed on the strip, a clear line was rapidly observed inLe-positive but not inLe-negative individuals. In contrast, a significant number of cancer patients with Lewis-negative phenotype were found to possess CA19-9, while they were specifically genotyped asLe/-. No contradictory results were observed in cancer patients (n = 315) with respect to their Lewis genotypes and CA19-9 levels.Conclusionsc59T>G occurred commonly in thelealleles could be specifically and rapidly identified by the present method. This method appeared to be relevant for selecting cancer patients to be diagnosed with CA19-9.
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人类路易斯组织血型系统的分子遗传学分析。
DOI：
10.1016/s0021-9258(19)62041-7
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Shoko NishiharaS;Hisashi NarimatsuSQ;Hiroko IwasakiS;Shin Yazawal;Suguru Akamatsull;Takao Ando;Taiko SenoSS;Ikuyo NarimatsugQ
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1982-12
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The Journal of biological chemistry
影响因子：
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通讯作者：
John L. MagnaniS;Bo Nilssong;Manfred BrockhausSV;David Zopfl;Zenon SteplewskiJI;Hilary Koprowskill;V. Ginsburg
从人类毛发和血液中进行组织血型 Lewis 基因分型
DOI：
--
发表时间：
1996
期刊：
The Japanese Journal of Human Genetics
影响因子：
--
作者：
S. Yazawa;Hitoshi Oh;T. Nakajima;O. Hosomi;S. Akamatsu;K. Kishi
通讯作者：
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DOI：
10.1073/pnas.90.5.1667
发表时间：
1993-03-01
影响因子：
11.1
作者：
CHERNY, DY;BELOTSERKOVSKII, BP;NIELSEN, PE
通讯作者：
NIELSEN, PE
在无血清培养基中生产单克隆抗体。
影响因子：
2.2
作者：
Chang,TH;Steplewski,Z;Koprowski,H
通讯作者：
Koprowski,H