The potential protective effect of monoclonal antibodies to gonococcal outer membrane protein IA.
The potential protective effect of monoclonal antibodies to gonococcal outer membrane protein IA.
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单克隆抗体对淋球菌外膜蛋白 IA 的潜在保护作用。
DOI：
10.1099/00221287-133-9-2639
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
J. Heckels
中科院分区：
文献类型：
--
作者：
M. Virji;J. Fletcher;K. Zak;J. Heckels
杂交细胞系产生的单克隆抗体针对淋球菌外膜蛋白IA。一种抗体，SM 101，识别25个测试菌株中的24个表达P. IA的菌株-其余的表现出相对较小的交叉反应性。竞争性放射免疫分析显示，每种抗体都能有效地抑制其他抗体的结合，表明所识别的表位非常接近。在体外试验中使用抗体来研究它们在保护免受淋球菌感染方面的潜在功效。在细胞毒性试验中，抗体对淋球菌攻击的上皮细胞提供了一定的保护。它们在补体存在下非常有效地杀菌，此外，对同源和异源菌株具有调理作用。交叉反应抗体SM 101在两种测定中均是最有效的抗体之一。结果表明，由抗体SM 101识别的P.IA上的保守表位是淋球菌表面上用于免疫预防的潜在有效靶点。
Hybrid cell lines were derived which produced monoclonal antibodies directed against gonococcal outer membrane protein IA. One antibody, SM101, recognized 24 P.IA-expressing strains out of 25 tested--the rest exhibited relatively less cross-reactivity. Competitive radioimmunoassays revealed that each antibody could effectively inhibit binding of the others, suggesting close proximity of the epitopes recognized. The antibodies were used in assays in vitro to investigate their potential efficacy in protection against gonococcal infection. In a cytotoxicity assay, the antibodies afforded some protection to epithelial cells challenged with gonococci. They were very effective at bactericidal killing in the presence of complement and, in addition, were opsonic for homologous and heterologous strains. The cross-reacting antibody, SM101, was one of the most effective in both assays. The results show that the conserved epitope on P.IA recognized by antibody SM101 is potentially an effective target on the gonococcal surface for immunoprophylaxis.
使用淋球菌外膜蛋白 I 的单克隆抗体对淋病奈瑟菌进行血清学分类。
DOI：
10.1093/infdis/150.1.44
发表时间：
1984
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Knapp,JS;Tam,MR;Nowinski,RC;Holmes,KK;Sandström,EG
通讯作者：
Sandström,EG
淋球菌膜蛋白：对其在发病机制中作用的推测。
DOI：
--
发表时间：
1983
期刊：
Progress in allergy
影响因子：
--
作者：
Blake,MS;Gotschlich,EC
通讯作者：
Gotschlich,EC