Mycoplasma hyopneumoniae transcription unit organization: genome survey and prediction.
Mycoplasma hyopneumoniae transcription unit organization: genome survey and prediction.
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DOI：
10.1093/dnares/dsr028
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发表时间：
2011-12
期刊：
影响因子：
--
通讯作者：
Schrank IS
中科院分区：
文献类型：
--
作者：
Siqueira FM;Schrank A;Schrank IS
猪肺炎支原体与猪呼吸道疾病有关。虽然在许多原核生物中基因的组织和调控是众所周知的，但对支原体的了解是有限的。本研究对猪肺炎支原体(7448、J和232)的三个菌株进行了比较分析，重点是基因组组织和用于开放阅读框架(ORF)簇(OC)鉴定的基因比较。基因组织分析表明，猪肺炎支原体7448和J有117个OCS和34个单一ORF，而在猪肺炎支原体232中有116个OCS和36个单一ORF。基因组比较显示，7448株和J株以及7448株和232株OCS具有高度的同步性和基因序列保守性。从猪肺炎支原体7448基因组中选取21个OCs进行逆转录-聚合酶链式反应(RT-PCR)实验，验证了我们的预测。由于内部启动子的存在，OC中的ORF子集可以独立转录。我们的结果表明，在猪肺炎支原体中，转录发生在上游启动子的‘跑跑式’中，从而形成大的ORF簇(从2到29个ORF在同一方向上)，表明了一个复杂的转录组织。
Mycoplasma hyopneumoniae is associated with swine respiratory diseases. Although gene organization and regulation are well known in many prokaryotic organisms, knowledge on mycoplasma is limited. This study performed a comparative analysis of three strains of M. hyopneumoniae (7448, J and 232), with a focus on genome organization and gene comparison for open read frame (ORF) cluster (OC) identification. An in silico analysis of gene organization demonstrated 117 OCs and 34 single ORFs in M. hyopneumoniae 7448 and J, while 116 OCs and 36 single ORFs were identified in M. hyopneumoniae 232. Genomic comparison revealed high synteny and conservation of gene order between the OCs defined for 7448 and J strains as well as for 7448 and 232 strains. Twenty-one OCs were chosen and experimentally confirmed by reverse transcription–PCR from M. hyopneumoniae 7448 genome, validating our prediction. A subset of the ORFs within an OC could be independently transcribed due to the presence of internal promoters. Our results suggest that transcription occurs in ‘run-on’ from an upstream promoter in M. hyopneumoniae, thus forming large ORF clusters (from 2 to 29 ORFs in the same orientation) and indicating a complex transcriptional organization.
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