State-of-the-art microscopy to understand islets of Langerhans: what to expect next?
State-of-the-art microscopy to understand islets of Langerhans: what to expect next?
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DOI：
10.1111/imcb.12450
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发表时间：
2021-05
影响因子：
4
通讯作者：
Giepmans BN
中科院分区：
文献类型：
--
作者：
de Boer P;Giepmans BN
朗格汉斯的发现和胰腺中胰岛的显微镜描述是发现胰岛素的关键步骤。在过去的150年里，胰岛生物学和1型糖尿病的许多发现都是使用强大的显微镜技术进行的。在过去的十年中，结合新的探针，动物和组织模型，应用新的生物传感器和自动化的光和电子显微镜方法和其他（亚）细胞成像模式已证明其潜力，了解β细胞（病理）生理条件下。从荧光水母到真实的实时活体功能成像的成像演变、自动化和数据处理的革命以及分析成像技术分辨率的提高正在融合。在这里，我们回顾了创新的方法，从新的角度研究细胞和分子在高时空分辨率和活的模型，解决胰岛生物学。这些细胞成像技术的广泛应用将在未来几年内揭示胰岛（异常）功能的原因/后果。创新的显微镜的方法，允许从新的角度来解决胰岛生物学研究细胞和分子在高时空分辨率和活的模型在这篇文章中进行审查。这些细胞成像技术的广泛应用将在未来几年内为胰岛功能（异常）的原因/后果提供新的线索。
The discovery of Langerhans and microscopic description of islets in the pancreas were crucial steps in the discovery of insulin. Over the past 150 years, many discoveries in islet biology and type 1 diabetes have been made using powerful microscopic techniques. In the past decade, combination of new probes, animal and tissue models, application of new biosensors and automation of light and electron microscopic methods and other (sub)cellular imaging modalities have proven their potential in understanding the beta cell under (patho)physiological conditions. The imaging evolution, from fluorescent jellyfish to real‐time intravital functional imaging, the revolution in automation and data handling and the increased resolving power of analytical imaging techniques are now converging. Here, we review innovative approaches that address islet biology from new angles by studying cells and molecules at high spatiotemporal resolution and in live models. Broad implementation of these cellular imaging techniques will shed new light on cause/consequence of (mal)function in islets of Langerhans in the years to come. Innovative microscopic approaches that allow to address islet biology from new angles by studying cells and molecules at high spatiotemporal resolution and in live models are reviewed in this article. Broad implementation of these cellular imaging techniques will shed new light into the cause/consequence of (mal)function of islet of Langerhans in the years to come.
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影响因子：
4.3
作者：
Delgadillo-Silva, Luis Fernando;Tsakmaki, Anastasia;Ninov, Nikolay
通讯作者：
Ninov, Nikolay
影响因子：
8.2
作者：
FOULIS, AK;LIDDLE, CN;WEIR, RS
通讯作者：
WEIR, RS
影响因子：
4.6
作者：
Buckley C;Carvalho MT;Young LK;Rider SA;McFadden C;Berlage C;Verdon RF;Taylor JM;Girkin JM;Mullins JJ
通讯作者：
Mullins JJ
影响因子：
8.2
作者：
Fava, E.;Dehghany, J.;Ouwendijk, J.;Mueller, A.;Niederlein, A.;Verkade, P.;Meyer-Hermann, M.;Solimena, M.
通讯作者：
Solimena, M.
影响因子：
16.6
作者：
Arrojo e Drigo, Rafael;Jacob, Stefan;Berggren, Per-Olof
通讯作者：
Berggren, Per-Olof