Discordant expression of miR-103/7 and pantothenate kinase host genes in mouse.

Discordant expression of miR-103/7 and pantothenate kinase host genes in mouse.
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DOI:
10.1016/j.ymgme.2010.07.016
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发表时间:
2010-10
影响因子:
3.8
通讯作者:
Hayflick, Susan J.
Hayflick, Susan J.
中科院分区:
生物学2区
文献类型:
--
作者:
Polster, Brenda J.;Westaway, Shawn K.;Nguyen, Thuy M.;Yoon, Moon Y.;Hayflick, Susan J.

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miR-103和miR-107是泛酸激酶基因承载的microrna,被认为可以调节细胞脂质代谢。microrna介导的调控是复杂的,可能影响宿主基因的表达,在同一途径中的相关酶,或明显不同的目标。通过qRT-PCR,我们证明了miR-103和miR-107的表达与小鼠组织中宿主泛酸激酶基因的表达无关。因此,miR-103/7家族为剖析宿主基因中的microRNA转录、加工和协调功能提供了一个有趣的模型。
miR-103 and miR-107, microRNAs hosted by pantothenate kinase genes, are proposed to regulate cellular lipid metabolism. microRNA-mediated regulation is complex, potentially affecting expression of the host gene, related enzymes within the same pathway, or apparently distinct targets. Using qRT-PCR, we demonstrate that miR-103 and miR-107 expression does not correlate with expression of host pantothenate kinase genes in mouse tissues. The miR-103/7 family thus provides an intriguing model for dissecting microRNA transcription, processing and coordinated function within host genes.
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