Detection of lipopolysaccharide-binding proteins on membranes of murine lymphocyte and macrophage-like cell lines.
Detection of lipopolysaccharide-binding proteins on membranes of murine lymphocyte and macrophage-like cell lines.
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鼠淋巴细胞和巨噬细胞样细胞系膜上脂多糖结合蛋白的检测。
DOI：
10.1111/j.1574-6968.1991.tb04257.x
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
E. Rietschel
中科院分区：
文献类型：
--
作者：
T. Kirikae;F. Kirikae;F. Schade;Masao Yoshida;S. Kondo;K. Hisatsune;S. Nishikawa;E. Rietschel
小鼠淋巴细胞和巨噬细胞样细胞系上存在的内毒素结合蛋白通过配基印迹和随后结合的内毒素的免疫学检测得到鉴定。用SDS-PAGE分离小鼠前B细胞系70Z/3的膜蛋白，将其转移到硝基纤维素上，与明尼苏达沙门氏菌突变株R595的脂多糖(MRE-LPS)孵育。用抗MRE-内毒素抗体检测与硝酸纤维素膜蛋白结合的内毒素，内毒素与70z/3细胞膜蛋白中的一种结合。该蛋白还原时为40 kDa，非还原时为45 kDa。用链霉蛋白酶处理70z/3细胞后，内毒素结合蛋白消失，表明其表面位置。过量的游离脂A代表了内毒素的生物活性区域，它抑制了MRE-内毒素与蛋白质的结合。在前B细胞系CYG8、B细胞系CYG101和小鼠T细胞系BW5147上也发现了这种内毒素结合蛋白。然而，在B细胞系CYG34和骨髓瘤细胞系P3-X63-Ag8.653上检测不到它。在这些细胞株上未检测到其他内毒素结合蛋白。在小鼠巨噬细胞样细胞系J774.1中，检测到两种内毒素结合蛋白，一种为40 kDa，另一种为80 kDa。这些结果表明，MRE-内毒素与淋巴细胞的40 kDa蛋白特异性结合，而在巨噬细胞中，它与40和80 kDa的两种内毒素结合蛋白有关。
Lipopolysaccharide-(LPS) binding proteins present on murine-lymphocyte and macrophage-like cell lines were identified by a ligand-blotting method and subsequent immunological detection of bound LPS. Membrane proteins of the murine-pre-B-cell line 70Z/3 were separated by SDS-PAGE, transferred electrophoretically onto nitrocellulose, and the blot was incubated with LPS of the Salmonella minnesota Re-mutant R595 (mRe-LPS). LPS bound to proteins on nitrocellulose was immunologically detected by anti-mRe-LPS antibodies; LPS was associated with one of the membrane proteins of 70Z/3 cells. This protein was 40 kDa under reducing and 45 kDa under non-reducing conditions, respectively. Treatment of 70Z/3 cells with pronase led to the disappearance of the LPS-binding protein indicating its surface location. Excess free lipid A, which represents the biologically active region of LPS, inhibited the binding of mRe-LPS to the protein. This LPS-binding protein was also identified on the pre-B-cell line CYG8, the B-cell line CYG101 and the murine-T-cell line BW5147. It was, however, not detectable on the B-cell line CYG34 and the myeloma-cell line P3-X63-Ag8.653. No other LPS-binding protein could be detected on these cell lines. In the murine-macrophage-like cell line J774.1, two LPS-binding proteins, one of 40 kDa and one of 80 kDa, were detected. These results indicate that mRe-LPS is specifically bound to a 40-kDa protein of lymphocytes, whereas in the case of macrophages it is associated with two LPS-binding proteins of 40 and 80 kDa.
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影响因子：
4.4
作者：
M. Lei;D. Morrison
通讯作者：
M. Lei;D. Morrison
影响因子：
56.9
作者：
SCHUMANN, RR;LEONG, SR;ULEVITCH, RJ
通讯作者：
ULEVITCH, RJ
影响因子：
56.9
作者：
WRIGHT, SD;RAMOS, RA;MATHISON, JC
通讯作者：
MATHISON, JC
DOI：
10.1016/s0021-9258(18)68109-8
发表时间：
1988-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Hampton;D. Golenbock;C. H. Raetz
通讯作者：
R. Hampton;D. Golenbock;C. H. Raetz
哺乳动物细胞上表达的特定脂多糖受体的情况。
影响因子：
3.8
作者：
Morrison,DC
通讯作者：
Morrison,DC