Consistent levels of A-to-I RNA editing across individuals in coding sequences and non-conserved Alu repeats.
Consistent levels of A-to-I RNA editing across individuals in coding sequences and non-conserved Alu repeats.
复制标题
DOI：
10.1186/1471-2164-11-608
复制
发表时间：
2010-10-28
期刊：
影响因子：
4.4
通讯作者：
Eisenberg E
中科院分区：
文献类型：
--
作者：
Greenberger S;Levanon EY;Paz-Yaacov N;Barzilai A;Safran M;Osenberg S;Amariglio N;Rechavi G;Eisenberg E
腺苷转肌苷（A-to-I） rna编辑是一种重要的转录后机制，发生在人类转录组的许多位点，主要是在Alu重复序列中。它已被证明在人类大脑的几个目标中具有一致的编辑水平，并在几种人类病理中发生改变。然而，到目前为止，人类个体在其他组织中编辑水平的可变性尚未得到研究。在这里，我们分析了32个皮肤样本，观察了编码序列中三个基因的A-to-I编辑水平和六个不同基因的Alu重复序列。我们在不同的个体和组织中观察到高度一致的编辑水平，不仅在编码目标中，而且令人惊讶的是，在非进化保守的Alu重复序列中也是如此。我们的研究结果表明，Alu元件的a -to- i rna编辑是一个严格调控的过程，因此，可能在灵长类动物进化过程中被招募用于转录后调控机制。
Adenosine to inosine (A-to-I) RNA-editing is an essential post-transcriptional mechanism that occurs in numerous sites in the human transcriptome, mainly within Alu repeats. It has been shown to have consistent levels of editing across individuals in a few targets in the human brain and altered in several human pathologies. However, the variability across human individuals of editing levels in other tissues has not been studied so far. Here, we analyzed 32 skin samples, looking at A-to-I editing level in three genes within coding sequences and in the Alu repeats of six different genes. We observed highly consistent editing levels across different individuals as well as across tissues, not only in coding targets but, surprisingly, also in the non evolutionary conserved Alu repeats. Our findings suggest that A-to-I RNA-editing of Alu elements is a tightly regulated process and, as such, might have been recruited in the course of primate evolution for post-transcriptional regulatory mechanisms.
登录
查看更多内容
影响因子：
56.9
作者：
Lev-Maor, G;Sorek, R;Ast, G
通讯作者：
Ast, G
影响因子：
11.4
作者：
Keegan, LP;Brindle, J;O'Connell, MA
通讯作者：
O'Connell, MA
影响因子：
4.5
作者：
Palladino, MJ;Keegan, LP;Reenan, RA
通讯作者：
Reenan, RA
影响因子：
56.9
作者：
BRUSA, R;ZIMMERMANN, F;SPRENGEL, R
通讯作者：
SPRENGEL, R
影响因子：
5.3
作者：
PATTERSON, JB;SAMUEL, CE
通讯作者：
SAMUEL, CE