A conserved CaM- and radial spoke associated complex mediates regulation of flagellar dynein activity.
A conserved CaM- and radial spoke associated complex mediates regulation of flagellar dynein activity.
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DOI：
10.1083/jcb.200703107
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发表时间：
2007-11-05
影响因子：
7.8
通讯作者：
Smith, Elizabeth F.
中科院分区：
文献类型：
--
作者：
Dymek, Erin E.;Smith, Elizabeth F.
对于几乎所有的纤毛和真核鞭毛，第二信使钙和环磷酸腺苷参与调节动力蛋白驱动的微管滑动来调节跳动。钙调蛋白（Calmodulin, CaM）定位于轴突，是参与运动调节的关键钙传感器。使用免疫沉淀和质谱，我们确定了一个CaM-containing complex的成员，参与调节动力蛋白的活性。该复合体包括鞭毛相关蛋白91 (FAP91)，它与AAT-1具有相当大的序列相似性，AAT-1最初在睾丸中被鉴定为a激酶锚定蛋白（AKAP）结合蛋白。FAP91直接与位于辐条基部的径向辐条蛋白3 （AKAP）相互作用。在微管滑动试验中，将针对FAP91产生的抗体添加到动力蛋白活性降低的突变轴突组中，将动力蛋白活性恢复到野生型水平。这些综合结果表明，凸轮和辐条相关复合物介导径向辐条和动力蛋白臂之间的调节信号。
For virtually all cilia and eukaryotic flagella, the second messengers calcium and cyclic adenosine monophosphate are implicated in modulating dynein- driven microtubule sliding to regulate beating. Calmodulin (CaM) localizes to the axoneme and is a key calcium sensor involved in regulating motility. Using immunoprecipitation and mass spectrometry, we identify members of a CaM-containing complex that are involved in regulating dynein activity. This complex includes flagellar-associated protein 91 (FAP91), which shares considerable sequence similarity to AAT-1, a protein originally identified in testis as an A-kinase anchor protein (AKAP)– binding protein. FAP91 directly interacts with radial spoke protein 3 (an AKAP), which is located at the base of the spoke. In a microtubule sliding assay, the addition of antibodies generated against FAP91 to mutant axonemes with reduced dynein activity restores dynein activity to wild-type levels. These combined results indicate that the CaM- and spoke-associated complex mediates regulatory signals between the radial spokes and dynein arms.
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DOI：
10.1083/jcb.98.1.97
发表时间：
1984-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kamiya R;Witman GB
通讯作者：
Witman GB
DOI：
10.1016/s0006-291x(74)80487-0
发表时间：
1974-01-01
影响因子：
3.1
作者：
BROKAW, CJ;JOSSLIN, R;BOBROW, L
通讯作者：
BOBROW, L
影响因子：
7.8
作者：
BROKAW, CJ
通讯作者：
BROKAW, CJ
影响因子：
7.8
作者：
Gardner, L C;O'Toole, E;Perrone, C A;Giddings, T;Porter, M E
通讯作者：
Porter, M E
影响因子：
56.9
作者：
NAITOH, Y;KANEKO, H
通讯作者：
KANEKO, H