Regressed three-dimensional capillary network and inhibited angiogenic factors in the soleus muscle of non-obese rats with type 2 diabetes.
Regressed three-dimensional capillary network and inhibited angiogenic factors in the soleus muscle of non-obese rats with type 2 diabetes.
复制标题
DOI：
10.1186/1743-7075-8-77
复制
发表时间：
2011-11-03
影响因子：
4.5
通讯作者：
Ishihara A
中科院分区：
文献类型：
--
作者：
Kondo H;Fujino H;Murakami S;Nagatomo F;Roy RR;Ishihara A
根据对组织横截面进行二维毛细血管分析获得的结果，糖尿病已被证明与骨骼肌微血管病变和毛细血管退化的高风险相关。我们可视化了非肥胖的2型糖尿病大鼠（Goto-Kakizaki， GK）比目鱼肌毛细血管网络的三维结构，并将其与对照组Wistar大鼠进行比较，以提供新的信息，例如毛细血管网络的毛细血管体积。此外，我们使用基于TaqMan探针的实时PCR检测了GK大鼠比目鱼肌中促血管生成和抗血管生成基因的表达水平。正如预期的那样，GK组的血浆葡萄糖水平高于对照组，胰岛素水平低于对照组。GK大鼠比目鱼肌毛细血管网络三维结构回归，毛细血管体积小于对照组。GK中促血管生成因子HIF-1α、KDR、Flt-1、ANG-1、Tie-2 mRNA表达水平低于对照组，而抗血管生成因子TSP-1 mRNA表达水平高于对照组。这些数据表明，促血管生成因子的减少和抗血管生成因子的增加可能在2型糖尿病诱导的肌肉循环并发症中起重要作用。
Based on findings obtained using two-dimensional capillary analyses on tissue cross-sections, diabetes has been shown to be associated with a high risk for microangiopathy and capillary regression in skeletal muscles. We visualized the three-dimensional architecture of the capillary networks in the soleus muscle of non-obese Goto-Kakizaki (GK) rats with type 2 diabetes and compared them with those of control Wistar rats to provide novel information, e.g., capillary volume, on the capillary networks. In addition, we examined pro- and anti-angiogenic gene expression levels in the soleus muscle of GK rats using TaqMan probe-based real-time PCR. As expected, plasma glucose levels were higher and insulin levels lower in GK than control rats. The three-dimensional architecture of the capillary networks was regressed and capillary volume was smaller in the soleus muscle of GK compared to control rats. The mRNA expression levels of the pro-angiogenic factors HIF-1α, KDR, Flt-1, ANG-1, and Tie-2 were lower, whereas the level of the anti-angiogenic factor TSP-1 was higher in GK than control rats. These data suggest that a decrease in pro-angiogenic and increase in anti-angiogenic factors may play an important role in type 2 diabetes-induced muscle circulatory complications.
登录
查看更多内容
影响因子：
9.8
作者：
Mathieu-Costello, O;Kong, A;Henry, RR
通讯作者：
Henry, RR
影响因子：
8.2
作者：
Adler, A. I.;Erqou, S.;Robinson, A. H. N.
通讯作者：
Robinson, A. H. N.
影响因子：
5.6
作者：
Ko, Seung-Hyun;Cao, Wenhong;Liu, Zhenqi
通讯作者：
Liu, Zhenqi
影响因子：
82.9
作者：
Jiménez, B;Volpert, OV;Bouck, N
通讯作者：
Bouck, N
影响因子：
4.8
作者：
Kivela, Riikka;Silvennoinen, Mika;Vihko, Veikko
通讯作者：
Vihko, Veikko