Isolation and characterization of H3N8 equine influenza A virus associated with the 2011 epizootic in Mongolia.
Isolation and characterization of H3N8 equine influenza A virus associated with the 2011 epizootic in Mongolia.
复制标题
DOI：
10.1111/irv.12069
复制
发表时间：
2013-09
影响因子：
4.4
通讯作者：
Gray GC
中科院分区：
文献类型：
--
作者：
Yondon M;Heil GL;Burks JP;Zayat B;Waltzek TB;Jamiyan BO;McKenzie PP;Krueger WS;Friary JA;Gray GC
马流感病毒(EIV)疫情大约每10年影响210万匹蒙古马，严重影响蒙古国的经济和游牧生活。2011年建立了一个积极的监测项目，以监测在蒙古马中传播的流感病毒。2011年1月至9月，从Töv、Khentii和Dundgoi艾马格(省)自由放养的马群的马身上采集了鼻拭子。用实时逆转录聚合酶链式反应(RRT-PCR)检测甲型流感病毒的存在。培养甲型流感阳性标本，扩增病毒，提取病毒RNA，扩增基因片段，进行Sanger测序。总共对745匹马进行了擦拭；大多数马没有临床疾病迹象。2011年7月，蒙古首都的马匹中出现了流感样疾病的报告，随后，监测工作进行了调整，以擦拭与流行性感冒有关的马匹。在5月、6月、8月和9月收集了34份RRT-PCR流感阳性病毒样本。三个样本检测到病毒。基因序列研究表明，这三株病毒都是相同的H3N8病毒。系统发育分析表明，该毒株与2007至2008年间在中亚流行的其他H3N8 EIV非常相似。由于大型蒙古马群似乎经常感染EIV流行性感冒，继续进行此类例行的马流感监测似乎是谨慎的做法。这样做将提供一个早期预警系统，如果出现新的病毒，有助于评估EIV是否正在越界感染人类，并提供数据来评估当前EIV疫苗的可能有效性。
Equine influenza virus (EIV) epizootics affect 2·1 million Mongolian horses approximately every 10 years and critically impact economy and nomadic livelihood of Mongolia. An active surveillance program was established in 2011 to monitor influenza viruses circulating among Mongolian horses. Nasal swabs were collected from horses in free‐ranging horse herds in Töv, Khentii, and Dundgovi aimags (provinces) from January to September 2011. Real‐time reversetranscriptase–polymerase chain reaction (rRT‐PCR) was used to determine the presence of influenza A virus. Influenza A‐positive specimens were cultured to amplify virus; viral RNA was extracted, and gene segments were amplified and sequenced by Sanger sequencing. A total of 745 horses were swabbed; most horses were without clinical signs of illness. In July 2011, reports of influenza‐like illnesses emerged among horses in Mongolia's capital, and subsequently, surveillance efforts were adjusted to swab horses associated with the epizootic. Thirty‐four specimens of rRT‐PCR influenza‐positive virus were collected in May, June, August, and September. Three specimens yielded detectable virus. Gene sequence studies suggested that all three isolates were identical H3N8 viruses. Phylogenetic analyses indicated the strain was very similar to other H3N8 EIVs circulating in central Asia between 2007 and 2008. As large Mongolian equine herds often seem to suffer from EIV epizootics, it seems prudent to continue such routine equine influenza surveillance. Doing so will provide an early warning system, should novel viruses emerge, help in assessing if EIV is crossing over to infect humans and provide data to assess the likely effectiveness of current EIV vaccines.
登录
查看更多内容
DOI：
10.1161/circoutcomes.111.960922
发表时间：
2011-09
期刊：
Circulation. Cardiovascular quality and outcomes
影响因子：
--
作者：
Allen NB;Diez-Roux A;Liu K;Bertoni AG;Szklo M;Daviglus M
通讯作者：
Daviglus M
影响因子：
16.2
作者：
Allard R;Leclerc P;Tremblay C;Tannenbaum TN
通讯作者：
Tannenbaum TN
影响因子：
4.4
作者：
Blumentals WA;Nevitt A;Peng MM;Toovey S
通讯作者：
Toovey S
影响因子：
120.7
作者：
Fry, AM;Shay, DK;Anderson, LJ
通讯作者：
Anderson, LJ
影响因子：
5.2
作者：
Andreyeva, T.;Michaud, P.-C.;van Soest, A.
通讯作者：
van Soest, A.