Developmental Regulation of Synaptotagmin I, II, III, and IV mRNAs in the Rat CNS
Developmental Regulation of Synaptotagmin I, II, III, and IV mRNAs in the Rat CNS
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大鼠 CNS 中突触结合蛋白 I、II、III 和 IV mRNA 的发育调节
DOI：
10.1523/jneurosci.17-04-01206.1997
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
B. Marquèze
中科院分区：
文献类型：
--
作者：
F. Berton;C. Iborra;J. Boudier;M. Seagar;B. Marquèze
突触结合蛋白I是一种丰富的突触囊泡蛋白，在介导Ca 2+触发的神经递质释放中具有重要功能。我们已经分析了四个神经突触结合蛋白异构体的分布在出生后发育的大鼠中枢神经系统的原位杂交。Synaptotagmin I，II，III和IV基因具有不同的时空表达模式，除了在小脑颗粒细胞中，在平行纤维/浦肯野细胞突触的形成过程中检测到的四个转录本。在整个开发过程中，突触结合蛋白I mRNA在大脑中广泛表达，而突触结合蛋白II转录本主要在脊髓中。在所有阶段，synaptotagmin III的mRNA表达均匀，在大多数神经元检查，虽然在一个低水平。Synaptotagmin I，II和III基因表达主要在发育过程中增加，并持续到成年期，反映了神经元的分化。相反，synaptotagmin IV的成绩单占主导地位，在围产期的发展在一个异质群体的神经元，随后表达均匀，在一个低水平。在新生大鼠海马CA 3区和下托区观察到强烈的标记，但在CA 1区没有。在大脑皮层，检测到层特异性标记，在细胞层V的高表达。只有少数浦肯野细胞簇被标记在小脑的绒球和副绒球。在脊髓中也观察到表达突触结合蛋白IV基因的异质性神经元。因此，我们推测突触结合蛋白IV可能在哺乳动物神经系统的发育中发挥作用。
Synaptotagmin I is an abundant synaptic vesicle protein that has an essential function in mediating Ca2+-triggered neurotransmitter release. We have analyzed the distribution of four neural synaptotagmin isoforms during postnatal development of the rat CNS by in situ hybridization. Synaptotagmin I, II, III, and IV genes have distinct patterns of spatiotemporal expression except in cerebellum granule cells, where the four transcripts were detected during the formation of parallel fiber/Purkinje cell synapses. Throughout development synaptotagmin I mRNAs were widely expressed in brain, whereas synaptotagmin II transcripts were predominant in spinal cord. At all stages synaptotagmin III mRNAs were expressed uniformly in most neurons examined, although at a low level. Synaptotagmin I, II, and III gene expressions mainly increased during development and persisted in adulthood, mirroring neuronal differentiation. Conversely, synaptotagmin IV transcripts were predominant during perinatal development in a heterogeneous population of neurons and subsequently were expressed uniformly at a low level. Intense labeling was observed in the hippocampal CA3 field and in the subiculum, but not in the CA1 field, of the newborn rat. In cerebral cortex, lamina-specific labeling was detected with a high expression in cell layer V. Only a small number of Purkinje cell clusters were labeled in the flocculus and paraflocculus of the cerebellum. Heterogeneous sets of neurons expressing synaptotagmin IV gene also were observed in spinal cord. We thus speculate that synaptotagmin IV may a play a role in the development of the mammalian nervous system.
DOI：
10.1073/pnas.92.13.5895
发表时间：
1995-06-20
影响因子：
11.1
作者：
HUDSON, AW;BIRNBAUM, MJ
通讯作者：
BIRNBAUM, MJ