Putative cross-kingdom horizontal gene transfer in sponge (Porifera) mitochondria.
Putative cross-kingdom horizontal gene transfer in sponge (Porifera) mitochondria.
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DOI：
10.1186/1471-2148-6-71
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发表时间：
2006-09-14
影响因子：
3.4
通讯作者：
Huchon, Dorothee
中科院分区：
文献类型：
--
作者：
Rot, Chagai;Goldfarb, Itay;Ilan, Micha;Huchon, Dorothee
后生动物的线粒体基因组通常是没有内含子的紧凑分子。仅在珊瑚和海葵（刺胞动物）中报告了该规则的例外情况，其中在 cox1 和 nad5 基因中发现了 I 组内含子。在这里，我们展示了几条证据，证明内含子也可以在海绵（Porifera）的线粒体中找到。对海绵 Tetilla sp. 线粒体 cox1 基因的 2,349 bp 片段进行了测序。 （螺虫纲）。该片段表明存在 1143 bp 内含子。与所有刺胞动物线粒体内含子类似，假定的内含子具有 I 组内含子特征。内含子存在于 cox1 基因中，编码假定的归巢核酸内切酶。为了确定该内含子在海绵中的分布，对海绵多样性的几个代表的 cox1 基因进行了测序。该内含子仅在海绵目螺藻中发现。对 COI 蛋白序列和内含子开放阅读框的系统发育分析表明，内含子可能是从真菌供体水平传播的。尽管在过去几年中经常从海绵中分离出海绵相关真菌，但人们对海绵相关真菌知之甚少。我们认为，真菌和海绵之间的共生关系促进了线粒体内含子的水平基因转移。众所周知，生态关系在基因组水平上具有影响。在这里，基于基因组分析提出了海绵和真菌之间的生态关系。
The mitochondrial genome of Metazoa is usually a compact molecule without introns. Exceptions to this rule have been reported only in corals and sea anemones (Cnidaria), in which group I introns have been discovered in the cox1 and nad5 genes. Here we show several lines of evidence demonstrating that introns can also be found in the mitochondria of sponges (Porifera). A 2,349 bp fragment of the mitochondrial cox1 gene was sequenced from the sponge Tetilla sp. (Spirophorida). This fragment suggests the presence of a 1143 bp intron. Similar to all the cnidarian mitochondrial introns, the putative intron has group I intron characteristics. The intron is present in the cox1 gene and encodes a putative homing endonuclease. In order to establish the distribution of this intron in sponges, the cox1 gene was sequenced from several representatives of the demosponge diversity. The intron was found only in the sponge order Spirophorida. A phylogenetic analysis of the COI protein sequence and of the intron open reading frame suggests that the intron may have been transmitted horizontally from a fungus donor. Little is known about sponge-associated fungi, although in the last few years the latter have been frequently isolated from sponges. We suggest that the horizontal gene transfer of a mitochondrial intron was facilitated by a symbiotic relationship between fungus and sponge. Ecological relationships are known to have implications at the genomic level. Here, an ecological relationship between sponge and fungus is suggested based on the genomic analysis.
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影响因子：
5.1
作者：
Hiort, J;Maksimenka, K;Bringmann, G
通讯作者：
Bringmann, G
影响因子：
14.9
作者：
Dalgaard, JZ;Klar, AJ;Mian, IS
通讯作者：
Mian, IS
影响因子：
3.9
作者：
ARNASON, U;GULLBERG, A;WIDEGREN, B
通讯作者：
WIDEGREN, B
影响因子：
3.9
作者：
Beaten, MJ;Roger, AJ;Cavalier-Smith, T
通讯作者：
Cavalier-Smith, T
影响因子：
4.1
作者：
Erpenbeck, D;McCormack, GP;van Soest, RWM
通讯作者：
van Soest, RWM