Demonstration of monoclonal EBV genomes in Hodgkin's disease and Ki-1-positive anaplastic large cell lymphoma by combined Southern blot and in situ hybridization.
Demonstration of monoclonal EBV genomes in Hodgkin's disease and Ki-1-positive anaplastic large cell lymphoma by combined Southern blot and in situ hybridization.
复制标题
通过结合 Southern 印迹和原位杂交证明霍奇金病和 Ki-1 阳性间变性大细胞淋巴瘤中的单克隆 EBV 基因组。
作者：
I. Anagnostopoulos;Hermann Herbst;G. Niedobitek;H. Stein
应用Southern印迹法检测了42例霍奇金病（HD）和22例Ki-1阳性间变性大细胞淋巴瘤（Ki-1 + ALC）中EB病毒（EBV）DNA。7例HD和1例Ki-1 + ALC淋巴瘤用EBV基因组内部重复区特异性探针检测呈阳性。随后，使用EBV末端区域DNA探针可以证明这些病毒基因组是单克隆起源的。原位杂交结果显示，在2例HD患者中，EBV感染的细胞具有明显的Hodgkin和Reed-Sternberg细胞形态，提示EBV与部分HD患者有直接的病理关系。
Forty-two cases of Hodgkin's disease (HD) and 22 cases of Ki-1-positive anaplastic large cell (Ki-1 + ALC) lymphoma were examined by Southern blotting for the presence of Epstein-Barr virus (EBV) DNA. Seven cases of HD and one case of Ki-1 + ALC lymphoma scored positive with a probe specific for the internal repetitive region of the EBV genome. Subsequently, these viral genomes could be demonstrated to be monoclonal in origin using an EBV terminal region DNA probe. In situ hybridization revealed that in two HD cases, the EBV infected cells had the distinct morphology of Hodgkin and Reed-Sternberg cells, thus suggesting a direct pathoetiological relationship between EBV and some cases of HD.
DOI：
10.1073/pnas.81.14.4510
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
FINGEROTH, JD;WEIS, JJ;FEARON, DT
通讯作者：
FEARON, DT