A Minimally Invasive Method for Intratracheal Instillation of Drugs in Neonatal Rodents to Treat Lung Disease.
A Minimally Invasive Method for Intratracheal Instillation of Drugs in Neonatal Rodents to Treat Lung Disease.
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微创气管内给药治疗新生啮齿动物肺部疾病。
DOI：
10.3791/61729
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发表时间：
2021-08-04
影响因子：
1.2
通讯作者：
Harijith, Anantha
中科院分区：
文献类型：
--
作者：
Sudhadevi, Tara;Ha, Alison W.;Harijith, Anantha
用直接滴入气管的药物治疗新生啮齿动物可以作为研究局部给药的影响的有价值的工具。这具有直接的转化影响，因为表面活性剂和药物被局部施用到肺部。尽管有许多文献描述了在治疗实验中对成年小鼠和大鼠进行微创经口插管，但在新生大鼠幼崽中缺乏这种方法。幼犬的口气管区域/咽部尺寸较小，使得喉腔（声带）的可视化变得困难，导致气管内药物输送的成功率存在差异。我们在此证明了对新生大鼠幼崽进行有效的口腔插管是一种无创伤、微创的技术，因此可用于连续给药。我们使用带有照明系统和放大镜的手术耳镜来观察新生大鼠的气管开口。然后使用连接到移液器尖端的 1 mL 注射器滴注药物。使用伊文思蓝染料给药证明了递送方法的准确性。该方法易于训练，可作为气管内滴注药物的有效方法。该方法还可用于施用接种物或试剂来模拟动物的疾病状况，也可用于各种肺部疾病的基于细胞的治疗策略。
Treatment of neonatal rodent with drugs instilled directly into the trachea could serve as a valuable tool to study the impact of a locally administered drug. This has direct translational impact because surfactant and drugs are administered locally into the lungs. Though the literature has many publications describing minimally invasive transoral intubation of adult mice and rats in therapeutic experiments, this approach in neonatal rat pups is lacking. The small size of orotracheal region/pharynx in the pups makes visualization of laryngeal lumen (vocal cords) difficult, contributing to the variable success rate of intratracheal drug delivery. We hereby demonstrate effective oral intubation of neonatal rat pup - a technique that is non-traumatic and minimally-invasive, so that it can be used for serial administration of drugs. We used an operating otoscope with an illumination system and a magnifying lens to visualize the tracheal opening of the rat neonates. The drug is then instilled using a 1 mL syringe connected to a pipette tip. The accuracy of the delivery method was demonstrated using Evans blue dye administration. This method is easy to get trained in and could serve as an effective way to instill drugs into trachea. This method could also be used for administration of inoculum or agents to simulate disease conditions in animals and, also, for cell-based treatment strategies for various lung diseases.
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DOI：
10.1016/j.bbi.2018.03.031
发表时间：
2018-05
期刊：
Brain, behavior, and immunity
影响因子：
--
作者：
Litvin DG;Dick TE;Smith CB;Jacono FJ
通讯作者：
Jacono FJ
影响因子：
12.4
作者：
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通讯作者：
Debyser, Zeger
影响因子：
2.4
作者：
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通讯作者：
Song, Chang-Woo
影响因子：
4.4
作者：
Grayson, Mitchell H.;Ramos, Madeleine S.;Holtzman, Michael J.
通讯作者：
Holtzman, Michael J.
影响因子：
6
作者：
Moreira, Alvaro;Winter, Caitlyn;Ahuja, Sunil
通讯作者：
Ahuja, Sunil