Oxygen Radical Generation and Acute Pancreatitis: Effects of Dibutyltin Dichloride/Ethanol and Ethanol on Rat Pancreas
Oxygen Radical Generation and Acute Pancreatitis: Effects of Dibutyltin Dichloride/Ethanol and Ethanol on Rat Pancreas
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氧自由基的产生与急性胰腺炎：二氯化二丁基锡/乙醇和乙醇对大鼠胰腺的影响
DOI：
10.1097/00006676-199511000-00010
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发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
W. Dummler
中科院分区：
文献类型：
--
作者：
H. Weber;J. Merkord;L. Jonas;A. Wagner;H. Schroder;U. Käading;A. Werner;W. Dummler
近年来的研究表明，氧自由基释放增加在急性胰腺炎的发病机制中起重要作用。因此，我们研究了用二丁基二氯化锡/乙醇（DBTCiEtOH：6 mg kg−1/13.7 mg kg−I，i. v.）处理的大鼠胰腺组织中产生氧自由基的黄嘌呤氧化酶（XOD）的活性，单独的乙醇（EtOH：13.7 mmol kg-1，i. v.），或等渗盐水（NaCl）作为对照。我们还研究了氧自由基清除剂超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPX）的活性。此外，还测定了脂质过氧化标记物丙二醛（MDA）的水平。未检测到XOD活性增强。处理后1和6 h，DBTC/EtOH比单独EtOH显著降低SOD活性，此后无差异。相应地，两种方案都降低了GPX活性。此外，DBTC/EtOH和EtOH在1小时内迅速增加MDA水平，指示给药后早期释放氧自由基。16 h后，仅DBTC 1 EtOH组MDA浓度仍升高。虽然在两组中观察到类似的代谢改变，但只有DBTCiEtOH在24 h内诱导急性间质性胰腺炎。我们的结论是：（a）组织中氧化剂和抗氧化剂之间的失衡可能在DBTCiEtOH诱导的急性间质性胰腺炎的发病机制中具有重要意义;（B）虽然EtOH增加了氧自由基水平，但急性胰腺炎的发展需要额外的损伤;（c）XOD似乎不负责显著的氧自由基产生; DBTC/EtOH模型是研究大鼠急性间质性胰腺炎的有效工具。
Recent studies suggest that enhanced release of free oxygen radicals plays an important role in the pathogenesis of acute pancreatitis. Therefore, we studied the activity of the oxygen radical generating xanthine ox-idase (XOD) in pancreatic tissue from rats treated with either dibutyltin dichloride/ethanol (DBTCiEtOH: 6 mg kg−1/13.7 mg kg−I, i.v.), ethanol alone (EtOH: 13.7 mmol kg−1, i.v.), or isotonic saline (NaCl) as control. We also investigated activities of the oxygen radical scavengers superoxide dismutase (SOD) and glutathione peroxidase (GPX). In addition, levels of the lipid peroxidation marker malondialdehyde (MDA) were determined. Enhanced activity of XOD was not detected. While SOD activity 1 and 6 h after treatment was significantly more reduced by DBTC/EtOH than by EtOH alone, no difference was found thereafter. Correspondingly, both regimens diminished GPX activity. Moreover, DBTC/EtOH and EtOH rapidly increased MDA levels within 1 h, in dicating release of oxygen radicals early on after administration. After 16 h the MDA concentration was still elevated only in the DBTCiEtOH group. Although similar metabolic alterations were observed in both groups, only DBTCiEtOH induced acute interstitial pancreatitis within 24 h. We conclude that (a) a tissue imbalance between oxidants and antioxidants might be of importance in the pathogenesis of DBTCiEtOH-induced acute interstitial pancreatitis; (b) although EtOH increases oxygen radical levels, additional damage is required for development of acute pancreatitis; (c) XOD does not seem to be responsible for significant oxygen radical generation; and (d) the DBTC/EtOH model is a useful tool to study acute interstitial pancreatitis in rats.
乙醇对正常和乙醇喂养大鼠胰腺腺泡淀粉酶分泌和细胞钙稳态的影响。
影响因子：
5.8
作者：
Ponnappa,BC;Hoek,JB;Waring,AJ;Rubin,E
通讯作者：
Rubin,E
影响因子：
2.9
作者：
SPITZ, DR;OBERLEY, LW
通讯作者：
OBERLEY, LW