Molecular analysis of Plasmodium ovale variants.

Molecular analysis of Plasmodium ovale variants.
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DOI:
10.3201/eid1007.030411
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发表时间:
2004-07
影响因子:
11.8
通讯作者:
Kawamoto F
Kawamoto F
中科院分区:
医学2区
文献类型:
--
作者:
Win TT;Jalloh A;Tantular IS;Tsuboi T;Ferreira MU;Kimura M;Kawamoto F

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6个东南亚分离株的序列分析支持疟原虫的分型获得了东南亚3个变异型和3个经典型卵形疟原虫分离株的小亚基核糖体RNA(SSUrRNA)基因的全序列和3个其它位点的部分序列,并与GenBank数据进行了比较。在每个变异型分离物中发现三个不同的SSURNA序列(Pov 1-3),在每个经典型分离物中发现两个不同的SSURNA序列(Poc 1-2)。Pov 1-3更接近于先前在喀麦隆和MAL/MAI分离株中发现的序列,而Poc 1-2更接近于先前在尼日利亚I/CDC菌株的两个克隆中发现的序列。Pov 1-3的3 ′半与来自伦敦学校(LS)菌株的SSUrRNA基因的部分序列相同。结果支持将卵形疟原虫分为两组,经典型(包括尼日利亚I/CDC菌株)和变异型(喀麦隆、MAL/MAI和LS分离株)。
Sequence analyses of six isolates from Southeast Asia supports dividing species into types Complete DNA sequences of the small subunit ribosomal RNA (SSUrRNA) gene and partial sequences of three other loci were obtained from three variant-type and three classic-type Plasmodium ovale isolates from Southeast Asia and compared with GenBank-available data. Three different SSUrRNA sequences (Pov 1–3) were found in each variant-type isolate, and two different SSUrRNA sequences (Poc 1–2) in each classic-type isolate. Pov 1–3 were closer to sequences previously found in the Cameroon and MAL/MAI isolates, whereas Poc 1–2 were closer to sequences previously found in two clones of the Nigerian I/CDC strain. The 3´ half of Pov 1–3 was identical to the partial sequence of the SSUrRNA gene from the London School (LS) strain. Results support grouping P. ovale into two groups, the classic type (including the Nigerian I/CDC strain) and the variant type (Cameroon, MAL/MAI, and LS isolates).
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