Characterization of a novel Manduca sexta beta-1, 3-glucan recognition protein (βGRP3) with multiple functions.
Characterization of a novel Manduca sexta beta-1, 3-glucan recognition protein (βGRP3) with multiple functions.
复制标题
DOI：
10.1016/j.ibmb.2014.06.003
复制
发表时间：
2014-09
影响因子：
3.8
通讯作者：
Yu, Xiao-Qiang
中科院分区：
文献类型：
--
作者：
Rao, Xiang-Jun;Zhong, Xue;Lin, Xin-Yu;Huang, Xiao-Hong;Yu, Xiao-Qiang
昆虫模式识别受体对病原体的识别是产生有效免疫应答的关键。本文报道了烟草天蛾β-1，3-葡聚糖识别蛋白（βGRP）家族的一个新成员β GRP 3。与M. sexta βGRP家族蛋白含有N-末端小葡聚糖结合结构域和C-末端大葡聚糖酶样结构域，β GRP 3在N-末端短40-45个残基并且缺乏小葡聚糖结合结构域。β GRP 3的葡聚糖酶样结构域与M. sexta微生物结合蛋白（MBP）的同源性为78%。β GRP 3主要在脂肪体中表达，其mRNA和蛋白水平均不受微生物诱导。从果蝇S2细胞中纯化的重组β GRP 3能与多种革兰氏阴性菌、革兰氏阳性菌和酵母菌结合，也能与海带多糖结合β-葡聚糖、甘露聚糖、脂多糖（LPS）、脂磷壁酸（LTA）和内消旋二氨基庚二酸（DAP）型肽聚糖（PG），但不与赖氨酸型PG结合。LPS和LTA的竞争，但几乎不被游离PG竞争。重组β GRP 3能以钙依赖的方式凝集蜡样芽孢杆菌和大肠杆菌，并对B具有抗菌（抑菌）活性。cereus中发现了βGRP家族蛋白的新功能。M. sexta β GRP 3可能是一种具有多种功能的免疫监视受体。
Recognition of pathogens by insect pattern recognition receptors is critical to mount effective immune responses. In this study, we reported a new member (βGRP3) of the β-1, 3-glucan recognition protein (βGRP) family from the tobacco hornworm Manduca sexta. Unlike other members of the M. sexta βGRP family proteins, which contain an N-terminal small glucan binding domain and a C-terminal large glucanase-like domain, βGRP3 is 40–45 residues shorter at the N-terminus and lacks the small glucan binding domain. The glucanase-like domain of β GRP3 is most similar to that of M. sexta microbe binding protein (MBP) with 78% identity. βGRP3 transcript was mainly expressed in the fat body, and both its mRNA and protein levels were not induced by microorganisms in larvae. Recombinant βGRP3 purified from Drosophila S2 cells could bind to several Gram-negative and Gram-positive bacteria and yeast, as well as to laminarin (β-1, 3-glucan), mannan, lipopolysaccharide (LPS), lipoteichoic acid (LTA), and meso-diaminopimelic acid (DAP)-type peptidoglycan (PG), but did not bind to Lysine-type PG. Binding of βGRP3 to laminarin could be competed well by free laminarin, mannan, LPS and LTA, but almost not competed by free PGs. Recombinant βGRP3 could agglutinate Bacillus cereus and Escherichia coli in a calcium-dependent manner and showed antibacterial (bacteriostatic) activity against B. cereus, novel functions that have not been reported for the βGRP family proteins before. M. sexta βGRP3 may serve as an immune surveillance receptor with multiple functions.
登录
查看更多内容
影响因子：
4.8
作者：
Kanagawa, Mayumi;Satoh, Tadashi;Yamaguchi, Yoshiki
通讯作者：
Yamaguchi, Yoshiki
影响因子：
4.8
作者：
Kim, YS;Ryu, JH;Lee, WJ
通讯作者：
Lee, WJ
影响因子：
2.9
作者：
Rao, Xiang-Jun;Yu, Xiao-Qiang
通讯作者：
Yu, Xiao-Qiang
影响因子：
3.8
作者：
Fabrick, JA;Baker, JE;Kanost, MR
通讯作者：
Kanost, MR
影响因子：
4.8
作者：
Mishima, Yumiko;Quintin, Jessica;Roussel, Alain
通讯作者：
Roussel, Alain