Lipoteichoic acid and lipopolysaccharide can activate antimicrobial peptide expression in the tobacco hornworm Manduca sexta.
Lipoteichoic acid and lipopolysaccharide can activate antimicrobial peptide expression in the tobacco hornworm Manduca sexta.
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DOI：
10.1016/j.dci.2010.06.007
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发表时间：
2010-10
影响因子：
2.9
通讯作者：
Yu, Xiao-Qiang
中科院分区：
文献类型：
--
作者：
Rao, Xiang-Jun;Yu, Xiao-Qiang
关键词：
酚氧化酶原的激活和抗菌肽的合成是昆虫体内两种重要的先天免疫机制。在本研究中，我们研究了三种主要细菌成分，即脂多糖(LPS)(包括LPS的粗略突变体)、脂磷壁酸(LTA)和肽聚糖(PG)对鳞翅目昆虫Manduca sexta幼虫的免疫应答。我们发现，大肠杆菌和枯草芽孢杆菌的两个DAP(二氨基甲酸)型PG在体外激活六分枝杆菌幼虫血浆中的酚氧化酶原的能力比各自细菌的LPS和LTA以及赖氨酸型PG强得多。光滑脂多糖(TLR4级)和粗面突变体(TLR4级™)、合成脂A、LTA和PG显著诱导幼虫血细胞和脂肪体中AMP基因的转录水平。大肠杆菌和枯草杆菌LTA的脂多糖对AMP表达的激活作用显著高于各自菌株的PG，光滑的脂多糖比脂类A和粗品突变体更能激活AMP的表达。我们的结果首次证明了LTA能够激活AMP的表达，不同含量的内毒素可能会协同激活AMP的表达。
Activation of prophenoloxidase and synthesis of antimicrobial peptides (AMPs) are two important innate immune mechanisms in insects. In the current study, we investigated immune responses activated by three major bacterial components, lipopolysaccharide (LPS) (including rough mutants of LPS), lipoteichoic acid (LTA), and peptidoglycan (PG), in the larvae of a lepidopteran insect, Manduca sexta. We found that two DAP (Diaminopimelic acid)-type PGs from Escherichia coli and Bacillus subtilis were much more potent than LPS and LTA from the respective bacteria as well as a Lysine-type PG in activation of prophenoloxidase in M. sexta larval plasma in vitro. Transcription levels of AMP genes, such as Attacin, Lebocin and Moricin genes, in the hemocytes and fat body of larvae were significantly induced by smooth LPS (TLR4grade) and rough mutants of LPS (TLRgrade™), synthetic lipid A, LTA, and PG. LPS from E. coli and LTA from B. subtilis activated AMP expression to significantly higher levels than PGs from the respective bacterial strains, and smooth LPS were more potent than lipid A and rough mutants of LPS in activation of AMP expression. Our results demonstrated for the first time that LTA can activate AMP expression, and different moieties of LPS may synergistically activate AMP expression in M. sexta.
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DOI：
10.1016/j.cbpb.2008.02.007
发表时间：
2008-06-01
影响因子：
2.2
作者：
Choi, Yong Soo;Choo, Young Moo;Jin, Byung Rae
通讯作者：
Jin, Byung Rae
DOI：
10.1073/pnas.0404952102
发表时间：
2005-01-25
影响因子：
11.1
作者：
Choe, KM;Lee, H;Anderson, KV
通讯作者：
Anderson, KV
影响因子：
3.1
作者：
Greenberg, JW;Fischer, W;Joiner, KA
通讯作者：
Joiner, KA
DOI：
10.1111/j.1432-1033.1997.00614.x
发表时间：
1997-07-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Axen, A;Carlsson, A;Bennich, H
通讯作者：
Bennich, H
影响因子：
3.6
作者：
Ao, Jing-qun;Ling, Erjun;Yu, Xiao-Qiang
通讯作者：
Yu, Xiao-Qiang