Cigarette Smoke Triggers Loss of Corneal Endothelial Cells and Disruption of Descemet's Membrane Proteins in Mice.
Cigarette Smoke Triggers Loss of Corneal Endothelial Cells and Disruption of Descemet's Membrane Proteins in Mice.
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香烟烟雾引发小鼠角膜内皮细胞损失和后弹力膜蛋白破坏。
DOI：
10.1167/iovs.62.3.3
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发表时间：
2021-03-01
影响因子：
4.4
通讯作者：
Riazuddin SA
中科院分区：
文献类型：
--
作者：
Ali M;Khan SY;Jang Y;Na CH;Talbot CC Jr;Gottsch JD;Handa JT;Riazuddin SA
探讨慢性香烟烟雾（CS）对小鼠角膜内皮细胞（CE）分子水平的影响。将妊娠小鼠（妊娠第18-20天）置于全身暴露吸烟室中，几天后出生幼仔。CS暴露3.5个月后，使用ConfoScan 4扫描显微镜检查CS暴露小鼠和对照（Ct）小鼠的角膜内皮细胞（CEC）。在显微镜下剥离CE，并对CS暴露小鼠和Ct小鼠进行4次生物学重复（2只雄性和2只雌性）;每次重复由16个CE组成。通过质谱法研究了CE的蛋白质组。CS暴露小鼠和Ct小鼠的CE图像显示CEC形状的差异，伴随着CS暴露后CEC密度降低近10%（P < 0.00003）。蛋白质组分析确定了共524个蛋白质表现出统计学显着的变化，CE从CS暴露的小鼠。重要的是，与德斯密氏膜（DM）相关的蛋白质，包括COL 4 α1、COL 4 α2、COL 4 α3、COL 4 α4、COL 4 α5、COL 4 α6、COL 8 α1、COL 8 α2和FN 1等，在CS暴露小鼠的CE中表现出降低的蛋白质水平。我们的数据证实，暴露于CS导致CEC密度降低，伴随着与DM相关的多种胶原蛋白和细胞外基质蛋白水平降低。
To investigate changes at a molecular level in the mouse corneal endothelium (CE) exposed to chronic cigarette smoke (CS). Pregnant mice (gestation days 18–20) were placed in a whole-body exposure smoking chamber, and a few days later pups were born. After 3.5 months of CS exposure, a ConfoScan4 scanning microscope was used to examine the corneal endothelial cells (CECs) of CS-exposed and control (Ct) mice. The CE was peeled under a microscope and maintained as four biological replicates (two male and two female) for CS-exposed and Ct mice; each replicate consisted of 16 CEs. The proteome of the CE was investigated through mass spectrometry. The CE images of CS-exposed and Ct mice revealed a difference in the shape of CECs accompanied by a nearly 10% decrease in CEC density (P < 0.00003) following CS exposure. Proteome profiling identified a total of 524 proteins exhibiting statistically significant changes in CE from CS-exposed mice. Importantly, proteins associated with Descemet's membrane (DM), including COL4α1, COL4α2, COL4α3, COL4α4, COL4α5, COL4α6, COL8α1, COL8α2, and FN1, among others, exhibited diminished protein levels in the CE of CS-exposed mice. Our data confirm that exposure to CS results in reduced CEC density accompanied by diminished levels of multiple collagen and extracellular matrix proteins associated with DM.
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影响因子：
3.7
作者：
Fujihara, Masashi;Nagai, Norihiro;Sussan, Thomas E.;Biswal, Shyam;Handa, James T.
通讯作者：
Handa, James T.
影响因子：
3.5
作者：
Gould, Douglas B.;Marchant, Jeffrey K.;John, Simon W. M.
通讯作者：
John, Simon W. M.
DOI：
10.1097/icl.0000000000000212
发表时间：
2016-09-01
影响因子：
2.3
作者：
Ilhan, Nilufer;Ilhan, Ozgur;Keskin, Ugurcan
通讯作者：
Keskin, Ugurcan
影响因子：
4.2
作者：
Rodahl, Eyvind;Knappskog, Per M.;Boman, Helge
通讯作者：
Boman, Helge
影响因子：
4.4
作者：
Jurkunas, Ula V.;Bitar, Maya S.;Joyce, Nancy C.
通讯作者：
Joyce, Nancy C.