A pig model of ischemic mitral regurgitation induced by mitral chordae tendinae rupture and implantation of an ameroid constrictor.
A pig model of ischemic mitral regurgitation induced by mitral chordae tendinae rupture and implantation of an ameroid constrictor.
复制标题
二尖瓣腱索断裂植入阿美罗缩肌引起缺血性二尖瓣关闭不全的猪模型
DOI：
10.1371/journal.pone.0111689
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Tang Y
中科院分区：
文献类型：
--
作者：
Cui YC;Li K;Tian Y;Yuan WM;Peng P;Yang JZ;Zhang BJ;Zhang HD;Wu AL;Tang Y
采用二尖瓣后腱索断裂植入ameroid缩窄器，建立了猪缺血性二尖瓣反流（IMR）的微型模型。一个2。采用x线冠状动脉造影、心电图分析、超声心动图和磁共振成像（MRI）评估猪在基线和术后1、2、4和8周的心脏结构和功能。发现二尖瓣反流的血流速度在中高水平之间。血管造影分析显示，植入ameroid缩窄器后一周LCX闭合率为10-20%，两周为30-40%，四周为90-100%。心电图分析显示，术后2周左心房（LA）直径增加，术后4周左心室（LV）和左室壁缺血改变。超声心动图和MRI进一步检测到术后两周左室和左室容积逐渐增加。与基线相比，术后猪心脏左端舒张和收缩容积以及左端舒张和收缩容积也显著升高。在心脏中观察到病理改变，包括左室中央缺血区瘢痕组织。透射电镜显示缺血区周围有收缩带和水肿，缺血区内有炎症细胞浸润。我们利用二尖瓣后腱索断裂技术和植入ameroid缩窄器建立了猪IMR模型。发现该猪IMR模型的病理特征与患者IMR的自然历史和进展相似。
A miniature pig model of ischemic mitral regurgitation (IMR) was developed by posterior mitral chordae tendinae rupture and implantation of an ameroid constrictor. A 2.5-mm ameroid constrictor was placed around the left circumflex coronary artery (LCX) of male Tibetan miniature pigs to induce ischemia, while the posterior mitral chordae tendinae was also ruptured. X-ray coronary angiography, ECG analysis, echocardiography, and magnetic resonance imaging (MRI) were used to evaluate heart structure and function in pigs at baseline and one, two, four and eight weeks after the operation. Blood velocity of the mitral regurgitation was found to be between medium and high levels. Angiographic analyses revealed that the LCX closure was 10–20% at one week, 30–40% at two weeks and 90–100% at four weeks subsequent ameroid constrictor implantation. ECG analysis highlighted an increase in the diameter of the left atria (LA) at two weeks post-operation as well as ischemic changes in the left ventricle (LV) and LA wall at four weeks post-operation. Echocardiography and MRI further detected a gradual increase in LA and LV volumes from two weeks post-operation. LV end diastolic and systolic volumes as well as LA end diastolic and systolic volume were also significantly higher in pig hearts post-operation when compared to baseline. Pathological changes were observed in the heart, which included scar tissue in the ischemic central area of the LV. Transmission electron microscopy highlighted the presence of contraction bands and edema surrounding the ischemia area, including inflammatory cell infiltration within the ischemic area. We have developed a pig model of IMR using the posterior mitral chordae tendineae rupture technique and implantation of an ameroid constrictor. The pathological features of this pig IMR model were found to mimic the natural history and progression of IMR in patients.
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影响因子：
37.8
作者：
Grigioni, F;Enriquez-Sarano, M;Tajik, AJ
通讯作者：
Tajik, AJ
DOI：
10.1111/j.0742-2822.2004.40027.x
发表时间：
2004-11-01
影响因子：
1.5
作者：
Khanna, D;Vengala, S;Misra, VK
通讯作者：
Misra, VK
影响因子：
2.8
作者：
FEHSKE, W;OMRAN, H;LUDERITZ, B
通讯作者：
LUDERITZ, B
影响因子：
37.8
作者：
Heegaard, Anne-Marie;Corsi, Alessandro;Bianco, Paolo
通讯作者：
Bianco, Paolo
DOI：
10.1016/j.vascn.2008.12.004
发表时间：
2009-07-01
影响因子：
1.9
作者：
Markert, Michael;Stubhan, Miriam;Guth, Brian
通讯作者：
Guth, Brian