The Bet v 1 fold: an ancient, versatile scaffold for binding of large, hydrophobic ligands.
The Bet v 1 fold: an ancient, versatile scaffold for binding of large, hydrophobic ligands.
复制标题
DOI：
10.1186/1471-2148-8-286
复制
发表时间：
2008-10-15
影响因子：
3.4
通讯作者：
Breiteneder, Heimo
中科院分区：
文献类型：
--
作者：
Radauer, Christian;Lackner, Peter;Breiteneder, Heimo
桦树花粉过敏原betv1是普遍存在的植物发病相关蛋白PR-10家族的一员。近年来，人们发现了许多与betv1序列相似度较低的植物蛋白。此外，对betv1结构的测定揭示了一个大的结构相关蛋白超家族的存在。在这项研究中，我们旨在从蛋白质数据库中识别和分类所有与Bet v 1相关的结构和Uniprot数据库中所有与Bet v 1相关的序列。将已知与betv1结构相关的蛋白质家族的代表性成员与蛋白质数据库的所有条目进行结构比较，得到47个序列不相同的结构。它们被分为11个家族，其中5个是新发现的，未被纳入蛋白质结构分类数据库1.71版。从Pfam蛋白家族数据库中提取的这些家族的分类分布表明，聚酮环化酶家族成员和Hsp90 atp酶同源物激活物1家族成员分布在所有三个超级王国中，而某些细菌家族成员仅限于少数物种。Bet v - 1样超家族成员的配体结合活性比较表明，它们的功能与脂类、激素和抗生素等大型疏水化合物的结合和代谢有关。betv1家族（定义为与betv1具有显著序列相似性的蛋白质组）的系统发育关系通过比对264个betv1相关序列来确定。基于距离的系统发育树将其分为11个亚科，9个完全包含植物序列和2个细菌蛋白质亚科。植物序列包括致病相关蛋白10、主要乳胶蛋白/成熟相关蛋白亚家族和聚酮环化酶样序列。Bet v1相关蛋白在所有超级王国中无处不在的分布表明，Bet v1样蛋白已经存在于最后的普遍共同祖先中。在进化过程中，这种蛋白质分化成许多具有低序列相似性的家族，但具有共同的折叠，成功地作为结合大体积配体的多功能支架。
The major birch pollen allergen, Bet v 1, is a member of the ubiquitous PR-10 family of plant pathogenesis-related proteins. In recent years, a number of diverse plant proteins with low sequence similarity to Bet v 1 was identified. In addition, determination of the Bet v 1 structure revealed the existence of a large superfamily of structurally related proteins. In this study, we aimed to identify and classify all Bet v 1-related structures from the Protein Data Bank and all Bet v 1-related sequences from the Uniprot database. Structural comparisons of representative members of already known protein families structurally related to Bet v 1 with all entries of the Protein Data Bank yielded 47 structures with non-identical sequences. They were classified into eleven families, five of which were newly identified and not included in the Structural Classification of Proteins database release 1.71. The taxonomic distribution of these families extracted from the Pfam protein family database showed that members of the polyketide cyclase family and the activator of Hsp90 ATPase homologue 1 family were distributed among all three superkingdoms, while members of some bacterial families were confined to a small number of species. Comparison of ligand binding activities of Bet v 1-like superfamily members revealed that their functions were related to binding and metabolism of large, hydrophobic compounds such as lipids, hormones, and antibiotics. Phylogenetic relationships within the Bet v 1 family, defined as the group of proteins with significant sequence similarity to Bet v 1, were determined by aligning 264 Bet v 1-related sequences. A distance-based phylogenetic tree yielded a classification into 11 subfamilies, nine exclusively containing plant sequences and two subfamilies of bacterial proteins. Plant sequences included the pathogenesis-related proteins 10, the major latex proteins/ripening-related proteins subfamily, and polyketide cyclase-like sequences. The ubiquitous distribution of Bet v 1-related proteins among all superkingdoms suggests that a Bet v 1-like protein was already present in the last universal common ancestor. During evolution, this protein diversified into numerous families with low sequence similarity but with a common fold that succeeded as a versatile scaffold for binding of bulky ligands.
登录
查看更多内容
Pfam：氏族、网络工具和服务。
影响因子：
14.9
作者：
Finn, Robert D.;Mistry, Jaina;Schuster-Bockler, Benjamin;Griffiths-Jones, Sam;Hollich, Volker;Lassmann, Timo;Moxon, Simon;Marshall, Mhairi;Khanna, Ajay;Durbin, Richard;Eddy, Sean R.;Sonnhammer, Erik L. L.;Bateman, Alex
通讯作者：
Bateman, Alex
影响因子：
5.1
作者：
FRISTENSKY, B;HOROVITZ, D;HADWIGER, LA
通讯作者：
HADWIGER, LA
DOI：
10.1046/j.1432-1327.1998.2580794.x
发表时间：
1998-12-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Fujimoto, Y;Nagata, R;Hashimoto, Y
通讯作者：
Hashimoto, Y
影响因子：
4.8
作者：
Mogensen, JE;Wimmer, R;Otzen, DE
通讯作者：
Otzen, DE
影响因子：
11.4
作者：
BREITENEDER, H;PETTENBURGER, K;BREITENBACH, M
通讯作者：
BREITENBACH, M