Accumulation and storage of ionized duplex DNA molecules in a quadrupole ion trap.
Accumulation and storage of ionized duplex DNA molecules in a quadrupole ion trap.
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四极离子阱中电离双链 DNA 分子的积累和储存。
DOI：
10.1021/ac00092a019
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发表时间：
1994
影响因子：
7.4
通讯作者：
McLuckey,SA
中科院分区：
文献类型：
--
作者：
Doktycz,MJ;Habibi-Goudarzi,S;McLuckey,SA
提出了四极离子阱中电离双链 DNA 分子积累和储存的证据。 DNA 互补单链分子的水溶液在溶液中退火形成双链体并进行电喷雾电离。在此过程中释放的离子通过大气/真空界面传输并注入四极离子阱，该四极离子阱使用压力为 1 mTorr 的浴气体操作。尽管相对于甲醇溶液中的单链寡核苷酸观察到的信号水平，水溶液电喷雾的信号水平大约差2个数量级，但使用水溶液以避免双链体变性。此处报告了由两个互补的 20 聚体单链和两个互补的 10 聚体组成的双链体的离子阱质谱。还报告了 10 聚体双链体的串联质谱结果。这些结果很重要，因为它们表明离子在相对较高的浴气体压力下操作的四极离子阱的离子注入、存储和质量分析条件下是动力学稳定的。因此，离子阱质谱分析工具可以应用于这一类重要的化合物。电喷雾电离在从溶液中的生物聚合物形成气态离子方面取得了显着的成功。 1 2**™ 5 事实上，它的受欢迎很大程度上源于其在生物医学研究中作为高性能分离和质谱分析接口的实用性。 6 电喷雾的一个特别有趣且迄今为止独特的特征是它能够形成具有生物相关性的非共价结合物种的气态离子，已知这些离子在物理条件下以特定复合物的形式存在。
Evidence for the accumulation and storage of ionized duplex DNA molecules in a quadrupole ion trap is presented. Aqueous solutions of complementary single-strand molecules of DNA were annealed toform duplexes in solution and subjected to electrospray ionization. The ions liberated in this process were transported through an atmosphere/vacuum interface and injected into a quadrupole ion trap operated with a bath gas present at a pressure of 1 mTorr. Despitethe roughly 2 order of magnitude poorer signal levels noted for electrospray of aqueous solutions relative to thoseobserved for single-strand oligonucleotides in methanol solutions, aqueous solutions were used to avoid denaturing the duplexes. Ion trap mass spectra are reported here for duplexes consisting of two complementary 20-mer single strands and two complementary 10-mers. Tandem mass spectrometry results are also reported for the 10-mer duplex. These results are significant in that they indicate that the ions are kinetically stable under the ion injection, storage, and mass analysis conditions of the quadrupole iontrap operated with a relatively high pressure of bath gas. The tools of ion trap mass spectrometry can therefore be applied to this important class of compounds.Electrospray ionization has enjoyed remarkable success in forming gaseous ions from biopolymers in solution. 1 2**™ 5 Indeed, much of its popularity derives from its utility in biomedical research as an interface for high-performance separations and mass spectrometry. 6 A particularly interesting, and so far unique, feature of electrospray is its ability to form gaseous ions of noncovalentlybound species of biological relevance that are known to exist as specific complexes under physi-
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影响因子：
15
作者：
LI, YT;HSIEH, YL;GANEM, B
通讯作者：
GANEM, B
电喷雾电离/离子阱质谱法测定嘧啶环丁烷二聚体
影响因子：
2.3
作者：
R. Ramsey;G. J. Berkel;S. A. McLuckey;G. Glish
通讯作者：
G. Glish
DOI：
10.1016/1044-0305(93)85026-t
发表时间：
1993-08-01
影响因子：
3.2
作者：
HUANG, EC;PRAMANIK, BN;COVEY, TR
通讯作者：
COVEY, TR
DOI：
10.1002/bms.1200220307
发表时间：
1993-03-01
期刊：
BIOLOGICAL MASS SPECTROMETRY
影响因子：
--
作者：
GOODLETT, DR;CAMP, DG;SMITH, RD
通讯作者：
SMITH, RD
使用轴向调制扩展四极离子阱的质量范围
影响因子：
2
作者：
R. Kaiser;J. N. Louris;J. Amy;R. Cooks;D. Hunt
通讯作者：
D. Hunt