Identification of melioidosis outbreak by multilocus variable number tandem repeat analysis.
Identification of melioidosis outbreak by multilocus variable number tandem repeat analysis.
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DOI：
10.3201/eid1502.081036
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发表时间：
2009-02
影响因子：
11.8
通讯作者：
Keim P
中科院分区：
文献类型：
--
作者：
Currie BJ;Haslem A;Pearson T;Hornstra H;Leadem B;Mayo M;Gal D;Ward L;Godoy D;Spratt BG;Keim P
对目录的一句话总结：该分析可在收到细菌分离株后8小时内确定该疾病的克隆爆发。地方性类鼻疽病是由基因多样化的假马利氏伯克氏菌菌株引起的。然而，克隆暴发（由一个菌株引起的多例）已经发生，例如来自受污染的饮用水。B. pseudomallei被指定为B类生物恐怖制剂，这需要迅速识别点源爆发。脉冲场凝胶电泳（PFGE）和多位点序列分型（MLST）可以鉴定遗传相关的分离株，但需要几天才能获得结果。我们开发了一种简化的4位点多位点可变数串联重复分析（MLVA-4），用于快速分型，并将结果与PFGE和MLST进行了比较。在相同的分离株中，MLVA-4优于MLST和PFGE；它区分了65种多位点序列类型，并显示了来自疫情聚集性的流行病学相关分离株和来自个体患者的分离株之间的相关性。MLVA-4可以证实或反驳在接受菌株后8小时内发生了类鼻瘤病的克隆爆发。
One-sentence summary for table of contents: This analysis can identify a clonal outbreak of this disease within 8 hours of receipt of bacterial isolates. Endemic melioidosis is caused by genetically diverse Burkholderia pseudomallei strains. However, clonal outbreaks (multiple cases caused by 1 strain) have occurred, such as from contaminated potable water. B. pseudomallei is designated a group B bioterrorism agent, which necessitates rapidly recognizing point-source outbreaks. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) can identify genetically related isolates, but results take several days to obtain. We developed a simplified 4-locus multilocus variable number tandem repeat analysis (MLVA-4) for rapid typing and compared results with PFGE and MLST for a large number of well-characterized B. pseudomallei isolates. MLVA-4 compared favorably with MLST and PFGE for the same isolates; it discriminated between 65 multilocus sequence types and showed relatedness between epidemiologically linked isolates from outbreak clusters and between isolates from individual patients. MLVA-4 can establish or refute that a clonal outbreak of melioidosis has occurred within 8 hours of receipt of bacterial strains.
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DOI：
10.4269/ajtmh.2004.71.360
发表时间：
2004-09-01
影响因子：
3.3
作者：
Gal, D;Mayo, M;Currie, BJ
通讯作者：
Currie, BJ
影响因子：
11.8
作者：
Rolim, DB;Vilar, DCFL;Inglis, TJJ
通讯作者：
Inglis, TJJ
影响因子：
9.4
作者：
Vesaratchavest, Mongkol;Tumapa, Sarinna;Peacock, Sharon J.
通讯作者：
Peacock, Sharon J.
影响因子：
9.4
作者：
Cheng, AC;Godoy, D;Currie, BJ
通讯作者：
Currie, BJ
影响因子：
9.4
作者：
Coenye, T;Spilker, T;LiPuma, JJ
通讯作者：
LiPuma, JJ