Effect of conformational features on the aminoacylation of tRNAs and consequences on the permutation of tRNA specificities.
Effect of conformational features on the aminoacylation of tRNAs and consequences on the permutation of tRNA specificities.
复制标题
构象特征对 tRNA 氨酰化的影响以及对 tRNA 特异性排列的影响。
DOI：
10.1016/0022-2836(92)90950-o
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发表时间：
1992
影响因子：
5.6
通讯作者：
R. Giegé
中科院分区：
文献类型：
--
作者：
V. Perret;C. Florentz;J. Puglisi;R. Giegé
体外转录的trnaasp变异体具有酵母tRNAPhe的构象特征，如可变区域的长度或d环中保守残基的排列，其结构和功能已经被研究。虽然它们在Pb2+处理下表现出显著的构象改变，但这些变异仍然可以被酵母天冬氨酸- trna合成酶有效地天冬氨酸化。因此，这种合成酶可以容纳多种tRNA构象。在第二组变异中，酵母trnahep的身份决定因子被移植到tRNAAsp先前的结构变异中。这些变体的苯丙氨酸接受度随着tRNAPhe结构特征数量的增加而提高，这表明苯丙酰- trna合成酶对嵌入同源同源核苷酸的构象框架很敏感。这些结果与tRNAAspidentity元件在酵母tRNAPhe中的有效移植形成对比。这表明合成酶对其tRNA底物的详细构象有不同的反应。有效的氨基酰化不仅依赖于一组同源核苷酸的存在，而且还依赖于tRNA的精确构象。
The structure and function ofin vitrotranscribed tRNAAspvariants with inserted conformational features characteristic of yeast tRNAPhe, such as the length of the variable region or the arrangement of the conserved residues in the D-loop, have been investigated. Although they exhibit significant conformational alterations as revealed by Pb2+treatment, these variants are still efficiently aspartylated by yeast aspartyl-tRNA synthetase. Thus, this synthetase can accommodate a variety of tRNA conformers. In a second series of variants, the identity determinants of yeast tRNAPhewere transplanted into the previous structural variants of tRNAAsp. The phenylalanine acceptance of these variants improves with increasing the number of structural characteristics of tRNAPhe, suggesting that phenylalanyl-tRNA synthetase is sensitive to the conformational frame embedding the cognate identity nucleotides. These results contrast with the efficient transplantation of tRNAAspidentity elements into yeast tRNAPhe. This indicates that synthetases respond differently to the detailed conformation of their tRNA substrates. Efficient aminoacylation is not only dependent on the presence of the set of identity nucleotides, but also on a precise conformation of the tRNA.
影响因子：
56.9
作者：
J. Sampson;A. Direnzo;L. Behlen;O. Uhlenbeck
通讯作者：
J. Sampson;A. Direnzo;L. Behlen;O. Uhlenbeck
DOI：
10.1073/pnas.85.4.1033
发表时间：
1988-02-01
影响因子：
11.1
作者：
SAMPSON, JR;UHLENBECK, OC
通讯作者：
UHLENBECK, OC
影响因子：
2.9
作者：
J. Sampson;A. Direnzo;L. Behlen;O. Uhlenbeck
通讯作者：
J. Sampson;A. Direnzo;L. Behlen;O. Uhlenbeck