Detection of α1-adrenoceptor subtypes in human hypertrophied prostate byin situ hybridizationsubtypes in human hypertrophied prostate byin situ hybridization
Detection of α1-adrenoceptor subtypes in human hypertrophied prostate byin situ hybridizationsubtypes in human hypertrophied prostate byin situ hybridization
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原位杂交检测人肥大前列腺中的α1-肾上腺素受体亚型 原位杂交检测人肥大前列腺中的α1-肾上腺素受体亚型
DOI：
10.1007/bf02409016
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
K. Kawabe
中科院分区：
文献类型：
--
作者：
N. Moriyama;S. Kurimoto;S. Horie;K. Nasu;Teruo Tanaka;K. Yano;H. Hirano;G. Tsujimoto;K. Kawabe
摘要肾上腺素能刺激通过α-1肾上腺素受体诱导肥大的前列腺组织收缩，药理学研究结果提示存在肾上腺素受体亚型。最近克隆了三个亚型(α1a、α1b和α1d)。利用这些亚型的探针，我们用原位杂交的方法证实了它们在10例良性前列腺增生症组织中的表达。为了确定这些亚型之间的比例，还对三个病例进行了核糖核酸酶保护试验。α-1a和α-1d肾上腺素能受体在间质中广泛表达，在腺上皮细胞中不表达。相反，α1b肾上腺素受体几乎检测不到。核糖核酸酶保护实验证实了α1b受体的缺失，α1a和α1d的比例为4：1。这些结果支持以下观点：几种肾上腺素能药物对前列腺收缩反应的差异是基于这些药物对不同亚型的亲和力。
SummaryAdrenergic stimulation induces contraction of hypertrophied prostatic tissue via the α1 adrenoceptor, and the results of pharmacological studies suggested the existence of adrenoceptor subtypes. Recently three subtypes (α1a, α1b, and α1d) were cloned. Using probes for these subtypes, we demonstrated their expression in the tissues of ten cases of benign prostatic hypertrophy, usingin situ hybridization. To determine the ratio between these subtypes, an RNase protection assay was also performed in three cases. Expression of the α1a and α1d adrenoceptors was diffuse in the smooth muscles of the interstitium, but was absent in glandular epithelial cells. On the contrary, the α1b adrenoceptor was hardly detectable. The RNase protection assay confirmed the absence of the α1b adrenoceptor, the ratio of α1a and α1d being 4∶1. These results supported the idea that the differences in prostatic contractile response to several adrenergic drugs are based on the affinities of these drugs for the different subtypes.
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人类 α1d-肾上腺素能受体的克隆以及 SK-N-MC 细胞中三种人类亚型的诱导表达。
DOI：
--
发表时间：
1995
期刊：
Molecular pharmacology.
影响因子：
--
作者：
Esbenshade,TA;Hirasawa,A;Tsujimoto,G;Tanaka,T;Yano,J;Minneman,KP;Murphy,TJ
通讯作者：
Murphy,TJ
大鼠脑中α1-肾上腺素能受体亚型的表征：[3H]WB4104 和[3H]哌唑嗪结合的重新评估。
影响因子：
3.6
作者：
Morrow,AL;Creese,I
通讯作者：
Creese,I
影响因子：
3.6
作者：
C. Forray;J. Bard;J. Wetzel;G. Chiu;E. Shapiro;R. Tang;H. Lepor;P. Hartig;R. Weinshank;T. Branchek
通讯作者：
C. Forray;J. Bard;J. Wetzel;G. Chiu;E. Shapiro;R. Tang;H. Lepor;P. Hartig;R. Weinshank;T. Branchek
影响因子：
3.6
作者：
MCVARY, KT;RAZZAQ, A;MCKENNA, KE
通讯作者：
MCKENNA, KE