The extrachromosomal circular DNAs of the rice blast pathogen Magnaporthe oryzae contain a wide variety of LTR retrotransposons, genes, and effectors.
The extrachromosomal circular DNAs of the rice blast pathogen Magnaporthe oryzae contain a wide variety of LTR retrotransposons, genes, and effectors.
复制标题
DOI：
10.1186/s12915-022-01457-2
复制
发表时间：
2022-11-23
期刊：
影响因子：
5.4
通讯作者：
Krasileva, Ksenia, V
中科院分区：
文献类型：
--
作者：
Joubert, Pierre M.;Krasileva, Ksenia, V
基因组对压力做出反应的方式之一是产生染色体外的环状DNA(EccDNA)。ECCDNA可以含有基因，并显著增加其拷贝数。它们还可以重新插入到基因组中，产生结构变异。它们已被证明在几个物种中提供了表型和遗传型可塑性的来源。然而，到目前为止，整个循环组的研究仅限于少数几个模式生物。真菌植物病原体对全球粮食安全构成严重威胁，部分原因是它们能迅速适应疾病预防策略。了解真菌病原体用来逃避疾病控制的机制对于遏制它们的威胁至关重要。我们对稻瘟病病原菌进行了全基因组测序研究。我们发现，水稻分枝杆菌有一个高度多样化的环体，其中包含许多基因，并显示出大量LTR反转录转座子活性的证据。我们发现水稻分枝杆菌eccDNA上富含的基因存在于易出现缺失变异的基因组区域，并且与疾病相关的基因经常位于eccDNA上。最后，我们发现在我们的数据中，eccDNA上永远不存在基因的子集，这表明eccDNA上这些基因的存在是相对于的。我们的研究为了解eccDNA如何促进米曲霉的适应奠定了基础。我们的分析还揭示了稻瘟病菌eccDNA与其他物种的eccDNA有何不同，并强调了需要进一步比较不同物种的eccDNA，以更好地了解这些分子。网上版载有补充材料，可在10.1186/s12915-022-01457-2查阅。
One of the ways genomes respond to stress is by producing extrachromosomal circular DNAs (eccDNAs). EccDNAs can contain genes and dramatically increase their copy number. They can also reinsert into the genome, generating structural variation. They have been shown to provide a source of phenotypic and genotypic plasticity in several species. However, whole circularome studies have so far been limited to a few model organisms. Fungal plant pathogens are a serious threat to global food security in part because of their rapid adaptation to disease prevention strategies. Understanding the mechanisms fungal pathogens use to escape disease control is paramount to curbing their threat. We present a whole circularome sequencing study of the rice blast pathogen, Magnaporthe oryzae. We find that M. oryzae has a highly diverse circularome that contains many genes and shows evidence of large LTR retrotransposon activity. We find that genes enriched on eccDNAs in M. oryzae occur in genomic regions prone to presence-absence variation and that disease-associated genes are frequently on eccDNAs. Finally, we find that a subset of genes is never present on eccDNAs in our data, which indicates that the presence of these genes on eccDNAs is selected against. Our study paves the way to understanding how eccDNAs contribute to adaptation in M. oryzae. Our analysis also reveals how M. oryzae eccDNAs differ from those of other species and highlights the need for further comparative characterization of eccDNAs across species to gain a better understanding of these molecules. The online version contains supplementary material available at 10.1186/s12915-022-01457-2.
登录
查看更多内容
影响因子：
5.3
作者：
Breen, James;Wicker, Thomas;Bellgard, Matthew
通讯作者：
Bellgard, Matthew
影响因子：
4.9
作者：
Bao W;Kojima KK;Kohany O
通讯作者：
Kohany O
影响因子：
4.6
作者：
Foster AJ;Martin-Urdiroz M;Yan X;Wright HS;Soanes DM;Talbot NJ
通讯作者：
Talbot NJ
影响因子：
4
作者：
Dong, Suomeng;Raffaele, Sylvain;Kamoun, Sophien
通讯作者：
Kamoun, Sophien
影响因子：
3.7
作者：
Galeote V;Bigey F;Beyne E;Novo M;Legras JL;Casaregola S;Dequin S
通讯作者：
Dequin S