Daily Suction Provided by External Volume Expansion Inducing Regeneration of Grafted Fat in a Murine Model
Daily Suction Provided by External Volume Expansion Inducing Regeneration of Grafted Fat in a Murine Model
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通过外部体积膨胀提供的每日吸力诱导小鼠模型中移植脂肪的再生
DOI：
10.1097/prs.0000000000003012
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发表时间：
2017-02
影响因子：
3.6
通讯作者：
Gao Jianhua
中科院分区：
文献类型：
--
作者：
Ye Yuan;Liao Yunjun;Lu Feng;Gao Jianhua
背景：脂肪移植的结果多种多样，有时效果不佳，因此需要新的方法。多项研究已经证明了体外扩容的出色表现，并且只关注预扩容，重点放在接受者身上。方法：建立两种小鼠脂肪移植模型(抽吸模型和脂肪交换移植模型)，观察在每日体外扩容吸力作用下移植脂肪再生相关细胞的来源和生物学行为的变化。结果：抽吸下新生的宿主来源的毛细血管或巨噬细胞的渗入增加了血供。CD34阳性细胞在吸引器作用下从宿主向移植物迁移增加。12周时，吸引组再生的成熟脂肪细胞有近一半来自宿主。在成脂过程中，在第2周和第4周，吸引组的过氧化物酶体增殖物激活受体的表达显著高于对照组。移植脂肪的标准化样本重量在第1周(0.081±0.001比0.072±0.005；p<；0.001)、4(0.060±0.002比0.048±0.001；p=0.002)、8(0.060±0.001vs0.046±0.001；p<；0.001)和12周(0.060±0.001比0.046±0.001；p=0.002)显著高于对照组。结论：体外扩容所提供的每日负压的机械作用有利于移植脂肪的再生，并通过促进再生相关细胞的迁移和脂肪细胞的分化来改善脂肪的滞留。因此，在吸力作用下，形成了更成熟的脂肪组织，结构整齐。
Background: Fat grafting has variable and sometimes poor outcomes, and therefore new methods are needed. Multiple studies have demonstrated the excellent performance of external volume expansion and focused only on preexpansion with emphasis on the recipient. Methods: Two mouse models (a suction model and a fat-exchange transplantation model) were established to investigate changes in the origins and biological behaviors of regeneration-related cells in grafted fat under daily suction provided by external volume expansion. Results: Blood supply increased from new host-derived capillaries or macrophage infiltration under suction. CD34-positive cells showed increased migration from the host into the grafts under suction. At week 12, nearly half of the mature adipocytes regenerated in the grafts in the suction group were derived from the host. Peroxisome proliferator-activated receptor &ggr; expression of the suction group was significantly higher than that of controls at weeks 2 and 4 during adipogenesis. The normalized sample weight of the grafted fat was significantly greater than that of controls at 1 (0.081 ± 0.001 versus 0.072 ± 0.005; p < 0.001), 4 (0.060 ± 0.002 versus 0.048 ± 0.001; p = 0.002), 8 (0.060 ± 0.001 versus 0.046 ± 0.001; p < 0.001), and 12 weeks (0.060 ± 0.001 versus 0.046 ± 0.001; p = 0.002). Conclusions: The mechanical effect of daily suction provided by external volume expansion favors the regeneration of grafted fat and improves retention by promoting the migration of regeneration-related cells and the differentiation of adipocytes. Thus, more mature fat tissue with a well-organized structure was formed under suction.
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影响因子：
2.3
作者：
Khouri, Roger;DelVecchio, Daniel
通讯作者：
DelVecchio, Daniel
DOI：
10.1016/j.yprs.2011.10.002
发表时间：
2013
期刊：
Yearbook of Plastic and Aesthetic Surgery
影响因子：
--
作者：
R. Ruberg
通讯作者：
R. Ruberg
影响因子：
3.6
作者：
R. Khouri;I. Schlenz;B. Murphy;T. J. Baker
通讯作者：
R. Khouri;I. Schlenz;B. Murphy;T. J. Baker
影响因子：
3.6
作者：
Heit, Yvonne I.;Lancerotto, Luca;Orgill, Dennis P.
通讯作者：
Orgill, Dennis P.
影响因子：
3.7
作者：
Chou WL;Galmozzi A;Partida D;Kwan K;Yeung H;Su AI;Saez E
通讯作者：
Saez E