YeastRGB: comparing the abundance and localization of yeast proteins across cells and libraries.
YeastRGB: comparing the abundance and localization of yeast proteins across cells and libraries.
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DOI：
10.1093/nar/gky941
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发表时间：
2019-01-08
影响因子：
14.9
通讯作者：
Levy ED
中科院分区：
文献类型：
--
作者：
Dubreuil B;Sass E;Nadav Y;Heidenreich M;Georgeson JM;Weill U;Duan Y;Meurer M;Schuldiner M;Knop M;Levy ED
测量酵母蛋白质组中蛋白质的丰度和可视化定位的能力激发了关于基因功能的假设并推动了发现。虽然经典的 C' 标记 GFP 酵母文库十多年来一直是唯一的资源，但最近 SWAT 技术的发展导致了多个新型酵母文库的创建，其中新一代荧光报告基因融合在开放阅读框的 N' 和 C' 处。有效访问这些数据需要一个用户界面来可视化和比较跨细胞、菌株和文库的蛋白质丰度、定位和共定位。 YeastRGB (www.yeastRGB.org) 旨在通过用户友好的界面最大限度地提高信息内容来满足这种需求。它采用紧凑型显示器，其中细胞被裁剪并平铺在一起形成“细胞网格”。这种表示方式可以在一个显示单元内查看特定菌株的数十个细胞，并且最多可以在标准高清屏幕上排列 30 个显示单元。此外，显示单元允许用户控制使用不同颜色通道获取的图像的缩放级别和叠加。因此，YeastRGB 可以有效地比较来自不同菌株和文库的数千个细胞的丰度和定位。
The ability to measure the abundance and visualize the localization of proteins across the yeast proteome has stimulated hypotheses on gene function and fueled discoveries. While the classic C’ tagged GFP yeast library has been the only resource for over a decade, the recent development of the SWAT technology has led to the creation of multiple novel yeast libraries where new-generation fluorescent reporters are fused at the N’ and C’ of open reading frames. Efficient access to these data requires a user interface to visualize and compare protein abundance, localization and co-localization across cells, strains, and libraries. YeastRGB (www.yeastRGB.org) was designed to address such a need, through a user-friendly interface that maximizes informative content. It employs a compact display where cells are cropped and tiled together into a ‘cell-grid.’ This representation enables viewing dozens of cells for a particular strain within a display unit, and up to 30 display units can be arrayed on a standard high-definition screen. Additionally, the display unit allows users to control zoom-level and overlay of images acquired using different color channels. Thus, YeastRGB makes comparing abundance and localization efficient, across thousands of cells from different strains and libraries.
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影响因子：
7.7
作者：
Lu AX;Chong YT;Hsu IS;Strome B;Handfield LF;Kraus O;Andrews BJ;Moses AM
通讯作者：
Moses AM
影响因子：
9.8
作者：
McQuin C;Goodman A;Chernyshev V;Kamentsky L;Cimini BA;Karhohs KW;Doan M;Ding L;Rafelski SM;Thirstrup D;Wiegraebe W;Singh S;Becker T;Caicedo JC;Carpenter AE
通讯作者：
Carpenter AE
影响因子：
6.8
作者：
Matalon, Or;Horovitz, Amnon;Levy, Emmanuel D.
通讯作者：
Levy, Emmanuel D.
DOI：
10.1534/g3.116.033654
发表时间：
2017-05-05
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Pärnamaa T;Parts L
通讯作者：
Parts L
影响因子：
48
作者：
Gordon, Andrew;Colman-Lerner, Alejandro;Brent, Roger
通讯作者：
Brent, Roger