Synapse-to-neuron ratio is inversely related to neuronal density in mature neuronal cultures.
Synapse-to-neuron ratio is inversely related to neuronal density in mature neuronal cultures.
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DOI：
10.1016/j.brainres.2010.08.058
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发表时间：
2010-11-04
期刊：
影响因子：
2.9
通讯作者：
Laplaca MC
中科院分区：
文献类型：
--
作者：
Cullen DK;Gilroy ME;Irons HR;Laplaca MC
突触形成是神经元中的一个基本过程，贯穿发育、成熟和衰老阶段。尽管这些阶段包含数量不同且不断变化的成熟神经元，但神经网络根据神经元密度来调节突触数量所采用的策略尚未被充分理解。本研究的目的是利用一种体外模型来评估细胞密度和神经元成熟度对突触数量和分布的影响。具体而言，大脑皮质神经元以每平方毫米10 - 5000个神经元的密度接种在平面培养物中，并在体外培养21天（DIV）期间通过免疫细胞化学评估突触数量和分布。高分辨率共聚焦显微镜显示，到体外培养21天，在高密度神经元网络的高度上，神经突起和突触呈现出精细的三维分布，其厚度可达18μm，这表明即使在平面高密度培养中也存在复杂程度的空间相互作用。在体外培养7天，每个神经元的平均突触数量少于5个，且这并不随神经元密度而变化。然而，到体外培养21天，每个神经元的突触数量跃升了30 - 80倍，并且与中等和较高神经元密度（每平方毫米500 - 4500个神经元；平均约150个突触/神经元）相比，较低神经元密度（<每平方毫米500个神经元；平均约400个突触/神经元）下的突触与神经元比率最大（p < 0.05）。这些结果表明神经元密度和突触数量之间存在一种关系，这可能对发育中的神经网络的神经生物学以及细胞死亡和再生过程具有影响。
Synapse formation is a fundamental process in neurons that occurs throughout development, maturity, and aging. Although these stages contain disparate and fluctuating numbers of mature neurons, tactics employed by neuronal networks to modulate synapse number as a function of neuronal density are not well understood. The goal of this study was to utilize an in vitro model to assess the influence of cell density and neuronal maturity on synapse number and distribution. Specifically, cerebral cortical neurons were plated in planar culture at densities ranging from 10 – 5000 neurons/mm2, and synapse number and distribution were evaluated via immunocytochemistry over 21 days in vitro (DIV). High-resolution confocal microscopy revealed an elaborate three-dimensional distribution of neurites and synapses across the heights of high-density neuronal networks by 21 DIV, which were up to 18 μm thick, demonstrating the complex degree of spatial interactions even in planar high-density cultures. At 7 DIV, the mean number of synapses per neuron was less than five, and this did not vary as a function of neuronal density. However, by 21 DIV, the number of synapses per neuron had jumped 30- to 80-fold, and the synapse-to-neuron ratio was greatest at lower neuronal densities (<500 neurons/mm2; mean approximately 400 synapses/neuron) compared to mid and higher neuronal densities (500 – 4500 neurons/mm2; mean approximately 150 synapses/neuron) (p < 0.05). These results suggest a relationship between neuronal density and synapse number that may have implications in the neurobiology of developing neuronal networks as well as processes of cell death and regeneration.
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影响因子：
3.3
作者：
EASTWOOD, SL;BURNET, PWJ;HARRISON, PJ
通讯作者：
HARRISON, PJ
DOI：
10.1083/jcb.101.4.1371
发表时间：
1985-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Binder LI;Frankfurter A;Rebhun LI
通讯作者：
Rebhun LI
影响因子：
5.3
作者：
Cancedda, Laura;Fiumelli, Hubert;Poo, Mu-ming
通讯作者：
Poo, Mu-ming
影响因子：
--
作者：
Boehler, Michael D.;Wheeler, Bruce C.;Brewer, Gregory J.
通讯作者：
Brewer, Gregory J.
影响因子：
2.4
作者：
LaPlaca, MC;Cullen, DK;Cargill, RS
通讯作者：
Cargill, RS