The distribution of tau in the mammalian central nervous system.
The distribution of tau in the mammalian central nervous system.
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DOI：
10.1083/jcb.101.4.1371
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发表时间：
1985-10
期刊：
影响因子：
--
通讯作者：
Rebhun LI
中科院分区：
文献类型：
--
作者：
Binder LI;Frankfurter A;Rebhun LI
我们利用一种能与牛和大鼠脑中所有的tau多肽结合的单克隆抗体，确定了异质性微管相关蛋白tau的生化和免疫细胞化学定位。通过免疫印迹分析和竞争性酶联免疫吸附测定，我们发现tau在牛脑白质提取物和微管中的含量比来自脑富集灰质区域的提取物和微管中的含量更为丰富。以每摩尔为基础，来自白质的两轮循环微管所含的tau是来自灰质的两轮循环微管的三倍。将tau的定位与微管相关蛋白2（MAP2）和微管蛋白的定位进行比较的免疫组织化学研究表明，tau仅限于轴突，这扩展了生化研究的结果。在神经胶质细胞中未观察到tau的定位，这表明至少在脑中，tau是神经元特异性的。这些观察结果表明，tau可能有助于确定一种仅限于轴突的微管亚群。此外，本报告中描述的单克隆抗体对于研究轴突萌发和生长的研究人员应该非常有用，因为它是一种独特的轴突标记物。
We have determined the biochemical and immunocytochemical localization of the heterogeneous microtubule-associated protein tau using a monoclonal antibody that binds to all of the tau polypeptides in both bovine and rat brain. Using immunoblot assays and competitive enzyme- linked immunosorbent assays, we have shown tau to be more abundant in bovine white matter extracts and microtubules than in extracts and microtubules from an enriched gray matter region of the brain. On a per mole basis, twice-cycled microtubules from white matter contained three times more tau than did twice-cycled microtubules from gray matter. Immunohistochemical studies that compared the localization of tau with that of MAP2 and tubulin demonstrated that tau was restricted to axons, extending the results of the biochemical studies. Tau localization was not observed in glia, which indicated that, at least in brain, tau is neuron specific. These observations indicate that tau may help define a subpopulation of microtubules that is restricted to axons. Furthermore, the monoclonal antibody described in this report should prove very useful to investigators studying axonal sprouting and growth because it is an exclusive axonal marker.
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DOI：
10.1073/pnas.79.16.4892
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
GINZBURG, I;SCHERSON, T;LITTAUER, UZ
通讯作者：
LITTAUER, UZ
影响因子：
2.5
作者：
BERNHARDT, R;MATUS, A
通讯作者：
MATUS, A
影响因子：
2.9
作者：
BENSADOUN, A;WEINSTEIN, D
通讯作者：
WEINSTEIN, D
DOI：
10.1083/jcb.98.3.1090
发表时间：
1984-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Drubin DG;Caput D;Kirschner MW
通讯作者：
Kirschner MW
影响因子：
3.7
作者：
CONNOLLY, JA;KALNINS, VI
通讯作者：
KALNINS, VI