A novel clinically translatable fluorescent nanoparticle for targeted molecular imaging of tumors in living subjects.
A novel clinically translatable fluorescent nanoparticle for targeted molecular imaging of tumors in living subjects.
复制标题
DOI：
10.1021/nl203526f
复制
发表时间：
2012-01-11
期刊：
影响因子：
10.8
通讯作者：
Cheng Z
中科院分区：
文献类型：
--
作者：
Gao J;Chen K;Luong R;Bouley DM;Mao H;Qiao T;Gambhir SS;Cheng Z
量子点(QD)在生物医学研究中的应用已经取得了巨大的增长，但由于许多临床问题，临床应用的成功例子还很少。在这里，我们报道了一种新型的稳定和生物相容的树枝状包覆的InP/ZnS核/壳量子点，作为一种临床上可翻译的分子成像应用的纳米探针。量子点(QD710-Dendron)具有几个显著的特征：近红外(NIR)发射，在生物介质中高度稳定，大小合适，可能具有肾脏清除和外渗能力。更重要的是，一项实验性的小鼠毒性研究证实，QD710-Dendron在测试的剂量下没有显著的毒性。QD710-Dendron的急性肿瘤摄取与周围非肿瘤组织形成良好的对比，表明QD710-Dendron被动靶向量子点的可能性。QD710-Dendron-RGD2对整合素αvβ3阳性肿瘤细胞的高度特异性靶向导致了肿瘤的高摄取率和纳米探针在肿瘤部位的长时间滞留。综上所述，QD710-Dendron和RGD修饰纳米粒具有小尺寸、高稳定性、生物相容性、良好的体内药代动力学和成功的肿瘤成像特性。这些功能满足了临床翻译的要求，并应推动在不久的将来进一步研究QD710-树状分子为基础的纳米探针在临床环境中使用的可能性。
The use of quantum dots (QDs) in biomedical research has grown tremendously, yet successful examples of clinical applications are absent due to many clinical concerns. Here, we report on a new type of stable and biocompatible dendron-coated InP/ZnS core/shell QDs as a clinically translatable nanoprobe for molecular imaging applications. The QDs (QD710-Dendron) were demonstrated to hold several significant features: near-infrared (NIR) emission, high stability in biological media, suitable size with possible renal clearance and ability of extravasation. More importantly, a pilot mouse toxicity study confirmed that QD710-Dendron lacks significant toxicity at the doses tested. The acute tumor uptake of QD710-Dendron resulted in good contrast from the surrounding non-tumorous tissues, indicating the possibility of passive targeting of the QDs. The highly specific targeting of QD710-Dendron-RGD2 to integrin αvβ3–positive tumor cells resulted in high tumor uptake and long retention of the nanoprobe at tumor sites. In summary, QD710-Dendron and RGD modified nanoparticles demonstrate small size, high stability, biocompatibility, favorable in vivo pharmacokinetics, and successful tumor imaging properties. These features satisfy the requirements for clinical translation and should promote efforts to further investigate the possibility of using QD710-Dendron based nanoprobes in the clinical setting in the near future.
登录
查看更多内容
影响因子：
3.4
作者：
Gao J;Chen X;Cheng Z
通讯作者：
Cheng Z
影响因子：
56.9
作者：
Bruchez, M;Moronne, M;Alivisatos, AP
通讯作者：
Alivisatos, AP
影响因子：
38.3
作者：
通讯作者：
--
影响因子：
14.8
作者：
Cai, Weibo;Chen, Xiaoyuan
通讯作者：
Chen, Xiaoyuan
影响因子：
16.6
作者：
Cheng, Zhiliang;Thorek, Daniel L. J.;Tsourkas, Andrew
通讯作者：
Tsourkas, Andrew