Development of Injectable Polydactyly-Derived Chondrocyte Sheets.
Development of Injectable Polydactyly-Derived Chondrocyte Sheets.
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DOI：
10.3390/ijms22063198
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发表时间：
2021-03-21
影响因子：
5.6
通讯作者：
Sato M
中科院分区：
文献类型：
--
作者：
Wasai S;Toyoda E;Takahashi T;Maehara M;Okada E;Uchiyama R;Akamatsu T;Watanabe M;Sato M
我们正在进行一项临床研究，利用同种异体多指软骨细胞片（PD片）修复骨关节炎引起的关节软骨损伤。然而，PD片的移植需要高侵入性的手术。为了建立一种侵入性较小的治疗方法，我们目前正在开发 PD 片的可注射片段（PD 片-迷你）。将多指软骨细胞接种到 RepCell™ 或传统的温度响应插入物中并进行培养。使用细胞计数和活力、组织学、酶联免疫吸附测定 (ELISA)、定量实时聚合酶链反应 (qPCR) 和流式细胞术来表征从每种培养物收集的 PD 片-迷你和 PD 片。为了检查注射对细胞活力的影响，在四种实验条件下测试了迷你 PD 片：非注射对照、18 号 (G) 针头、23G 针头和仅注射器。迷你PD片产生与PD片相似数量的体液因子。 PD片和PD片-迷你之间没有观察到组织学差异。除COL2A1外，两种类型的PD片之间软骨相关基因的表达没有差异。注射条件之间没有观察到显着差异。 PD 片-mini 具有类似于 PD 片的特性。 PD Sheets-mini 的细胞活力不受针规尺寸的显着影响。关节内注射可能是一种可行的、侵入性较小的微型PD片移植方法。
We are conducting a clinical study of the use of allogeneic polydactyly-derived chondrocyte sheets (PD sheets) for the repair of articular cartilage damage caused by osteoarthritis. However, the transplantation of PD sheets requires highly invasive surgery. To establish a less invasive treatment, we are currently developing injectable fragments of PD sheets (PD sheets-mini). Polydactyly-derived chondrocytes were seeded in RepCell™ or conventional temperature-responsive inserts and cultured. Cell counts and viability, histology, enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qPCR), and flow cytometry were used to characterize PD sheets-mini and PD sheets collected from each culture. To examine the effects of injection on cell viability, PD sheets-mini were tested in four experimental conditions: non-injection control, 18 gauge (G) needle, 23G needle, and syringe only. PD sheets-mini produced similar amounts of humoral factors as PD sheets. No histological differences were observed between PD sheets and PD sheets-mini. Except for COL2A1, expression of cartilage-related genes did not differ between the two types of PD sheet. No significant differences were observed between injection conditions. PD sheets-mini have characteristics that resemble PD sheets. The cell viability of PD sheets-mini was not significantly affected by needle gauge size. Intra-articular injection may be a feasible, less invasive method to transplant PD sheets-mini.
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