Autoinducer-2-Producing Protein LuxS, a Novel Salt- and Chloride-Induced Protein in the Moderately Halophilic Bacterium Halobacillus halophilus
Autoinducer-2-Producing Protein LuxS, a Novel Salt- and Chloride-Induced Protein in the Moderately Halophilic Bacterium Halobacillus halophilus
复制标题
自诱导剂 2 产生蛋白 LuxS，中度嗜盐细菌嗜盐芽孢杆菌中的一种新型盐和氯诱导蛋白
DOI：
--
复制
发表时间：
2006
影响因子：
4.4
通讯作者：
V. Müller
中科院分区：
文献类型：
--
作者：
Xaver Sewald;Stephan H. Saum;P. Palm;F. Pfeiffer;D. Oesterhelt;V. Müller
ABSTRACT The moderately halophilic bacterium Halobacillus halophilus carries a homologue of LuxS, a protein involved in the activated methyl cycle and the production of autoinducer-2, which mediates quorum sensing between certain species. luxS of H. halophilus is part of an operon that encodes an S-adenosylmethionine-dependent methyltransferase, a cysteine synthase, and a β-cystathionine lyase. Expression of luxS was growth phase dependent, with maximal expression in the mid-exponential growth phase. In addition, transcription of luxS was strictly salt dependent; maximal mRNA concentrations were observed with 2.0 M NaCl in the growth medium. Chloride ions stimulated luxS transcription by a factor of three. Western blot analyses demonstrated a growth phase- and salinity-dependent production of LuxS. Moreover, cellular LuxS levels were strictly chloride dependent. Maximal accumulation of LuxS was observed at 0.5 to 1.0 M Cl− and depended on the salinity.
利用脂质体、单克隆抗体和补体对霉菌毒素 T-2 进行均相免疫测定。
影响因子：
2.9
作者：
Ligler,FS;Bredehorst,R;Talebian,A;Shriver,LC;Hammer,CF;Sheridan,JP;Vogel,CW;Gaber,BP
通讯作者：
Gaber,BP
影响因子：
3.8
作者：
Daines, DA;Bothwell, M;Smith, AL
通讯作者：
Smith, AL