LncRNA and mRNA profiling during activation of tilapia macrophages by HSP70 and Streptococcus agalactiae antigen.
LncRNA and mRNA profiling during activation of tilapia macrophages by HSP70 and Streptococcus agalactiae antigen.
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HSP70 和无乳链球菌抗原激活罗非鱼巨噬细胞期间的 LncRNA 和 mRNA 分析
DOI：
10.18632/oncotarget.21427
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发表时间：
2017-11-17
期刊：
影响因子：
--
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Luo H;Yang H;Lin Y;Zhang Y;Pan C;Feng P;Yu Y;Chen X
研究罗非鱼腹膜巨噬细胞(TPM)激活过程中lncRNA的变化，探讨lncRNA与mRNA的关系。用RNA测序的方法研究无乳链球菌(Sa)抗原、热休克蛋白70(HSP70)和HSP70+Sa刺激后TPM激活的LncRNA和mRNA谱。用定量聚合酶链式反应(QPCR)检测LncRNA和mRNA的表达。通过配对比较，发现356个LncRNA、10173个m RNA和1 782个转录本存在差异表达。与mRNAs相比，这些LncRNAs的长度更短，外显子数量更少，表达水平更高。683个lncRNAs和4320个mRNAs共定位，316个lncRNAs和9997个mRNAs在共表达网络中。7个mRNAs(ANKRD34A、FMODA、GJA3、CNTN5、BMP10、BAI2和HS3ST6)参与了LNC_00035和LNC_000466的两个网络。差异表达基因涉及“磷酸化”、“细胞因子-细胞因子受体相互作用”、“内吞作用”和“MHC蛋白复合体”等信号转导途径。LNC_000792、LNC_000215、LNC_000035和LNC_000310与mRNAs有顺式和/或反式关系，也参与CERNA网络。这些结果可能代表了第一个被HSP70和Sa激活的罗非鱼巨噬细胞中lncRNAs和mRNAs的表达谱。
To investigate the lncRNA profiling during tilapia peritoneal macrophages (TPMs) activation and discuss the relationship between lncRNA and mRNA. RNA sequencing was used to investigate the lncRNA and mRNA profiles of TPMs activation following stimulation with Streptococcus agalactiae (Sa) antigen, heat shock protein 70 (HSP70) and HSP70+Sa. The expressions of lncRNA and mRNA were confirmed by qPCR. 356 lncRNA, 10173 mRNA and 1782 transcripts of uncertain coding potential (TUCP) were differentially expressed by pairwise comparison. These lncRNAs were shorter in length, fewer in exon number and higher in expression levels as compared with mRNAs. 683 lncRNAs and 4320 mRNAs were co-located, while 316 lncRNAs and 9997 mRNAs were in co-expression networks. Seven mRNAs (ANKRD34A, FMODA, GJA3, CNTN5, BMP10, BAI2 and HS3ST6) were involved in both networks of LNC_00035 and LNC_000466. Differentially expressed genes were involved in signaling pathways, such as “phosphorylation”, “cytokine-cytokine receptor interaction”, “endocytosis” and “MHC protein complex”. LNC_000792, LNC_000215, LNC_000035 and LNC_000310, with cis and/or trans relationships with mRNAs, were also involved in ceRNA network. These results might represent the first identified expression profile of lncRNAs and mRNAs in tilapia macrophages activated by HSP70 and Sa.
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影响因子：
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作者：
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通讯作者：
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