A newly discovered protein export machine in malaria parasites.
A newly discovered protein export machine in malaria parasites.
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DOI：
10.1038/nature08104
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发表时间：
2009-06-18
期刊：
影响因子：
64.8
通讯作者：
Crabb, Brendan S.
中科院分区：
文献类型：
--
作者：
de Koning-Ward, Tania F.;Gilson, Paul R.;Boddey, Justin A.;Rug, Melanie;Smith, Brian J.;Papenfuss, Anthony T.;Sanders, Paul R.;Lundie, Rachel J.;Maier, Alexander G.;Cowman, Alan F.;Crabb, Brendan S.
数百种疟原虫蛋白质被输出到包裹的空泡之外并进入宿主红细胞的胞质溶胶中，这一过程是致病疟原虫物种的毒性和生存能力的关键。负责这一出口的贩运机制尚不清楚。在这里，我们确定了疟原虫易位蛋白（PTEX），这是位于液泡膜。PTEX复合物是ATP驱动的，并且包含HSP 101（其是通常与蛋白质translocons相关的类型的ClpA/B样AAA+ ATP酶）、称为PTEX 150的新蛋白质和已知的寄生虫蛋白EXP 2。EXP 2是潜在的通道，因为它是核心PTEX复合物的膜相关组分。另外两种蛋白质，一种新的蛋白质PTEX 88和一种称为TRX 2的硫氧还蛋白，也被鉴定为PTEX组分。作为许多关键过程的共同门户，这种新的translocon为治疗干预提供了一个令人兴奋的新途径。
Several hundred malaria parasite proteins are exported beyond an encasing vacuole and into the cytosol of the host erythrocyte, a process that is key to the virulence and viability of the causative Plasmodium species. The trafficking machinery responsible for this export is unknown. Here, we identify a Plasmodium Translocon of EXported proteins (PTEX), which is located in the vacuole membrane. The PTEX complex is ATP-powered and comprises HSP101, which is a ClpA/B-like AAA+ ATPase of a type commonly associated with protein translocons, a novel protein termed PTEX150 and a known parasite protein EXP2. EXP2 is the potential channel as it is the membrane-associated component of the core PTEX complex. Two other proteins, a novel protein PTEX88 and a thioredoxin known as TRX2, were also identified as PTEX components. As a common portal for numerous crucial processes, this novel translocon offers an exciting new avenue for therapeutic intervention.
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