Accurate localization of linear probe electrodes across multiple brains
Accurate localization of linear probe electrodes across multiple brains
复制标题
跨多个大脑的线性探针电极的精确定位
DOI：
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
K. Svoboda
中科院分区：
文献类型：
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作者：
Liu D Liu;Susu Chen;M. N. Economo;Nuo Li;K. Svoboda
最近开发的硅探针在长的线性柄上具有大量的记录电极。具体而言，Neuropixels探针具有分布在9.6 mm柄上的960个记录电极。由于它们的长度，神经像素探测记录在啮齿动物自然跨越多个大脑区域。典型的研究整理了几个记录会话和动物的记录。在不同阶段和动物中记录的神经元必须相互对齐，并与标准化的大脑坐标系对齐。在这里，我们报告了一个工作流程，用于在标准化坐标中准确定位单个电极，并在单个大脑中对齐。该工作流程依赖于使用荧光探针轨迹对大脑进行成像，并将三维图像堆栈扭曲为标准化的大脑图谱。然后，电生理学特征被用于将特定电极锚沿着沿着重建的轨迹到脑图谱中的特定位置，并因此锚定到特定的脑结构。我们进行了地面实况实验，其中运动皮层输出用ChR2和荧光蛋白标记。这些输出所针对的大脑区域的记录显示电极定位的精度优于100 μm。
Recently developed silicon probes have large numbers of recording electrodes on long linear shanks. Specifically, Neuropixels probes have 960 recording electrodes distributed over 9.6 mm shanks. Because of their length, Neuropixels probe recordings in rodents naturally span multiple brain areas. Typical studies collate recordings across several recording sessions and animals. Neurons recorded in different sessions and animals have to be aligned to each other and to a standardized brain coordinate system. Here we report a workflow for accurate localization of individual electrodes in standardized coordinates and aligned across individual brains. This workflow relies on imaging brains with fluorescent probe tracks and warping 3-dimensional image stacks to standardized brain atlases. Electrophysiological features are then used to anchor particular electrodes along the reconstructed tracks to specific locations in the brain atlas and therefore to specific brain structures. We performed ground-truth experiments, in which motor cortex outputs are labelled with ChR2 and a fluorescence protein. Recording from brain regions targeted by these outputs reveals better than 100 μm accuracy for electrode localization.
影响因子：
2.5
作者：
Csicsvari, J;Henze, DA;Buzsáki, G
通讯作者：
Buzsáki, G
影响因子：
2.5
作者：
Henze, DA;Borhegyi, Z;Buzsáki, G
通讯作者：
Buzsáki, G